Human TLR7 Antibody Summary
Met360-Leu516
Accession # Q9NYK1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TLR7 in RPMI8226 Human Cell Line by Flow Cytometry. RPMI8226 human myeloma cell line was stained with Mouse Anti-Human TLR7 Monoclonal Antibody (Catalog # MAB5875, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
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Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry. Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 Monoclonal Antibody (Catalog # MAB5875, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TLR7
Toll-like receptor 7 (TLR7) is a 120 kDa (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. Human TLR7 is synthesized as a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 803 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid pre-dendritic cells. Functionally, TLR7 participates in the innate immune response to microbial agents.
Product Datasheets
Citations for Human TLR7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Functionally impaired plasmacytoid dendritic cells and non-haematopoietic sources of type I interferon characterize human autoimmunity
Authors: A Psarras, A Alase, A Antanavici, IM Carr, MY Md Yusof, M Wittmann, P Emery, GC Tsokos, EM Vital
Nature Communications, 2020-12-01;11(1):6149.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TLR7 in B cells promotes renal inflammation and Gd-IgA1 synthesis in IgA nephropathy
Authors: N Zheng, K Xie, H Ye, Y Dong, B Wang, N Luo, J Fan, J Tan, W Chen, X Yu
JCI Insight, 2020-07-23;5(14):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Satellite glial cells in human trigeminal ganglia have a broad expression of functional Toll-like receptors
Authors: JG Mitterreit, WJD Ouwendijk, M van Velzen, GP van Nierop, ADME Osterhaus, GMGM Verjans
Eur. J. Immunol., 2017-06-01;0(0):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Flow Cytometry
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Gated on CD45+ PBMCs
