Human TLR8 Antibody

Catalog #: MAB8999
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8999
MAB8999-SP

Detection of TLR8 in HEK293 Human Cell Line Transfected with Human TLR8 and eGFP by Flow Cytometry.

1 Image

All TLR8 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TLR8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TLR8 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 935166

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TLR8
Glu27-Thr827
Accession # Q9NR97

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of TLR8 in HEK293 Human Cell Line Transfected with Human TLR8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TLR8 and eGFP was stained with and either (A) Mouse Anti-Human TLR8 Monoclonal Antibody (Catalog # MAB8999) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR8

TLR8, also designated as CD288 (cluster of differentiation 288), is a member of the toll-like receptor (TLR) family. TLRs make up a family of pattern recognition receptors that play important roles in the innate immune response. Broad classes of pathogens (e.g. viruses, bacteria, and fungi) constitutively express a set of mutation-resistant molecules called pathogen-associated molecular patterns (PAMPs). These microbial molecular markers may be composed of proteins, carbohydrates, lipids, nucleic acids and/or combinations thereof. Individual TLRs recognize distinct pathogen-associated PAMPs, initiating signaling cascades that promote the immune response. Structurally, TLRs are type I transmembrane receptors that possess varying numbers of extracellular N-terminal leucine-rich repeat (LRR) motifs, followed by a cysteine-rich region, a TM domain, and an intracellular Toll/IL-1 R (TIR) motif. The TIR motif is common to the larger IL-1 R/TLR superfamily. Human TLR8 is an endosomal receptor that recognizes single stranded RNA (ssRNA), and can recognize ssRNA viruses such as Influenza, Sendai, and Coxsackie B viruses. TLR8 binding to the viral RNA recruits MyD88 and leads to activation of the transcription factor NF-kB and an antiviral response.

Long Name

Toll-like Receptor 8

Entrez Gene IDs

51311 (Human); 170744 (Mouse)

Alternate Names

CD288 antigen; CD288; MGC119599; MGC119600; TLR8; toll-like receptor 8

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human TLR8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1