Human TMIGD2/CD28H Alexa Fluor® 350-conjugated Antibody

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FAB8316U-100UG
R&D Systems Antibodies
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Human TMIGD2/CD28H Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TMIGD2/CD28H in direct ELISAs.  Stains human TMIGD2/CD28H transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2b Clone # 953728
Immunogen
HEK293 human embryonic kidney cell line-derived human TMIGD2/CD28H
Met1-Gly150
Accession # Q96BF3
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 human embryonic kidney cell line transfected with human TMIGD2/CD28H and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

References
  1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
  2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
  3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
  4. Huang, C. et al. (2003) Nature 424:219.
  5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.
Long Name
Transmembrane And Immunoglobulin Domain Containing 2
Entrez Gene IDs
126259 (Human)
Alternate Names
CD28H; IGPR1; IGPR-1; MGC23244; TMIGD2; transmembrane and immunoglobulin domain containing 2; transmembrane and immunoglobulin domain-containing protein 2

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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