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Human TNF-alpha Biotinylated Antibody

Catalog #: BAF210
26 Citations 1 Review Datasheet / COA / SDS
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BAF210
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TNF‑ alpha in Human PBMCs.
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Citations (26)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TNF-alpha Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) TNF‑ beta, recombinant mouse (rm) TNF-alpha, recombinant rat TNF‑ alpha, recombinant porcine TNF-alpha, rhsTNF RII, rmsTNF RI, and rmsTNF RII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TNF-alpha (R&D Systems, Catalog # 210-TA)
Val77-Leu233
Accession # P01375
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunocytochemistry
5-15 µg/mL
See below

Human TNF-alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human TNF‑ alpha Antibody (Catalog # MAB610)

Capture Reagent: Human TNF‑ alpha Antibody (Catalog # MAB610R)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TNF-a antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

TNF‑ alpha in Human PBMCs. TNF-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF-a Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF210) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF- is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

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Citations for Human TNF-alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Defining T cell receptor repertoires using nanovial-based binding and functional screening
    Authors: Koo, D;Mao, Z;Dimatteo, R;Noguchi, M;Tsubamoto, N;McLaughlin, J;Tran, W;Lee, S;Cheng, D;de Rutte, J;Burton Sojo, G;Witte, ON;Di Carlo, D;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human hepegivirus
    Sample Types: Cell Culture Supernates
    Applications: Capture Assay
  2. Effect of probiotic Lactobacillus paracasei on tumor necrosis factor-alpha level in gingival crevicular fluid of patients undergoing impacted third molar removal
    Authors: S Banjonjit, M Taweechoti, S Rungsiyano
    Journal of oral science, 2022-04-08;0(0):.
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
    Applications: ELISA Detection
  3. A conformation-selective monoclonal antibody against a small molecule-stabilised signalling-deficient form of TNF
    Authors: DJ Lightwood, RJ Munro, J Porter, D McMillan, B Carrington, A Turner, A Scott-Tuck, ES Hickford, A Schmidt, D Fox, A Maloney, T Ceska, T Bourne, J O'Connell, ADG Lawson
    Nature Communications, 2021-01-25;12(1):583.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (detection)
  4. Hydrogel based protein biochip for parallel detection of biomarkers for diagnosis of a Systemic Inflammatory Response Syndrome (SIRS) in human serum
    Authors: A Stumpf, T Brandstett, J Hübner, J Rühe
    PLoS ONE, 2019-12-02;14(12):e0225525.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  5. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017-04-14;12(4):e0175712.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  6. Transmembrane TNF-dependent uptake of anti-TNF antibodies
    Authors: A Deora, S Hegde, J Lee, CH Choi, Q Chang, C Lee, L Eaton, H Tang, D Wang, D Lee, M Michalak, M Tomlinson, Q Tao, N Gaur, B Harvey, S McLoughlin, B Labkovsky, T Ghayur
    MAbs, 2017-03-21;0(0):0.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  7. Porphyromonas gingivalis gingipains cause defective macrophage migration towards apoptotic cells and inhibit phagocytosis of primary apoptotic neutrophils
    Authors: SA Castro, R Collighan, PA Lambert, IH Dias, P Chauhan, CE Bland, I Milic, MR Milward, PR Cooper, A Devitt
    Cell Death Dis, 2017-03-02;8(3):e2644.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Elevated Membrane and Soluble CD64: A Novel Marker Reflecting Altered FcgammaR Function and Disease in Early Rheumatoid Arthritis That Can Be Regulated by Anti-Rheumatic Treatment.
    Authors: Matt P, Lindqvist U, Kleinau S
    PLoS ONE, 2015-09-25;10(9):e0137474.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  9. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011-07-09;54(10):2713-23.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  10. Protection from RNA and DNA viruses by IL-32.
    Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
    J. Immunol., 2011-02-23;186(7):4110-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  11. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  12. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  13. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  14. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  15. A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
    Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
    Clin. Exp. Allergy, 2008-02-25;38(4):594-601.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  16. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
    Authors: Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
    J. Immunol., 2008-02-15;180(4):2553-61.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  17. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007-11-01;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  18. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  19. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  20. Sepsis and pathophysiology of anthrax in a nonhuman primate model.
    Authors: Stearns-Kurosawa DJ, Lupu F, Taylor FB, Kinasewitz G, Kurosawa S
    Am. J. Pathol., 2006-08-01;169(2):433-44.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Plasma
    Applications: ELISA Development
  21. Selective regulation of IL-10 signaling and function by zymosan.
    Authors: Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB
    J. Immunol., 2006-04-15;176(8):4785-92.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  22. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
    Applications: ELISA Development
  23. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  24. IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
    Authors: Sharif MN, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv LB
    J. Immunol., 2004-05-15;172(10):6476-81.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  25. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004-01-01;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  26. Reprogramming of IL-10 activity and signaling by IFN-gamma.
    Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB
    J. Immunol., 2003-11-15;171(10):5034-41.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Human TNF-alpha Biotinylated Antibody
By Leslie Priddy on 04/13/2018
Application: IHC Sample Tested: Tumor cell lyastes Species: Human