Home / TNF RI/TNFRSF1A / Human TNF RI/TNFRSF1A Antibody

Human TNF RI/TNFRSF1A Antibody

Catalog #: MAB225
28 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB225-SP
MAB225-100
MAB225-500
TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody.
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Citations (28)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TNF RI/TNFRSF1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human (rh) TNF RII, rmTNF RI, or rmTNF RII.
Source
Monoclonal Mouse IgG1 Clone # 16803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize TNF RI/TNFRSF1A-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.008-0.04 µg/mL in the presence of 15 ng/mL Recombinant Human TNF RI/TNFRSF1A, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. View Larger

TNF RI/TNFRSF1A Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RI/TNFRSF1A Antibody. Recombinant Human TNF RI/TNFRSF1A (Catalog # 372-R1) inhibits Recombinant Human TNF-a (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RI/TNFRSF1A (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225). The ND50 is typically 0.008-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Flow Cytometry Detection of TNF RI/TNFRSF1A antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB225, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Citations for Human TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. An integrated organoid omics map extends modeling potential of kidney disease
    Authors: Lassé, M;El Saghir, J;Berthier, CC;Eddy, S;Fischer, M;Laufer, SD;Kylies, D;Hutzfeldt, A;Bonin, LL;Dumoulin, B;Menon, R;Vega-Warner, V;Eichinger, F;Alakwaa, F;Fermin, D;Billing, AM;Minakawa, A;McCown, PJ;Rose, MP;Godfrey, B;Meister, E;Wiech, T;Noriega, M;Chrysopoulou, M;Brandts, P;Ju, W;Reinhard, L;Hoxha, E;Grahammer, F;Lindenmeyer, MT;Huber, TB;Schlüter, H;Thiel, S;Mariani, LH;Puelles, VG;Braun, F;Kretzler, M;Demir, F;Harder, JL;Rinschen, MM;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry/ Immunofluorescence
  2. Dynamic bioinspired coculture model for probing ER+ breast cancer dormancy in the bone marrow niche
    Authors: L Pradhan, D Moore, EM Ovadia, SL Swedzinski, T Cossette, RA Sikes, K van Golen, AM Kloxin
    Science Advances, 2023-03-08;9(10):eade3186.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. TNF-alpha Affects Signature Cytokines of Th1 and Th17 T Cell Subsets through Differential Actions on TNFR1 and TNFR2
    Authors: B Pesce, CH Ribeiro, M Larrondo, V Ramos, L Soto, D Catalán, JC Aguillón
    International Journal of Molecular Sciences, 2022-08-18;23(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Plasmacytoid dendritic cells have divergent effects on HIV infection of initial target cells and induce a pro-retention phenotype
    Authors: O Tong, G Duette, TR O'Neil, CM Royle, H Rana, B Johnson, N Popovic, S Dervish, MAE Brouwer, H Baharlou, E Patrick, G Ctercteko, S Palmer, E Lee, E Hunter, AN Harman, AL Cunningham, N Nasr
    PloS Pathogens, 2021-04-19;17(4):e1009522.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. A conformation-selective monoclonal antibody against a small molecule-stabilised signalling-deficient form of TNF
    Authors: DJ Lightwood, RJ Munro, J Porter, D McMillan, B Carrington, A Turner, A Scott-Tuck, ES Hickford, A Schmidt, D Fox, A Maloney, T Ceska, T Bourne, J O'Connell, ADG Lawson
    Nature Communications, 2021-01-25;12(1):583.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1
    Authors: J Sellau, M Groneberg, H Fehling, T Thye, S Hoenow, C Marggraff, M Weskamm, C Hansen, S Stanelle-B, S Kuehl, J Noll, V Wolf, NG Metwally, SH Hagen, C Dorn, J Wernecke, H Ittrich, E Tannich, T Jacobs, I Bruchhaus, M Altfeld, H Lotter
    Nat Commun, 2020-07-10;11(1):3459.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  7. The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis
    Authors: Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su
    Oncogene, 2019-10-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Human TNF-Luc reporter mouse: A new model to quantify inflammatory responses
    Authors: F Minshawi, MRH White, W Muller, N Humphreys, D Jackson, BJ Campbell, A Adamson, S Papoutsopo
    Sci Rep, 2019-01-17;9(1):193.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Low doses of LPS exacerbate the inflammatory response and trigger death on TLR3-primed human monocytes
    Authors: M Monguió-To, M Franquesa, MR Sarrias, FE Borràs
    Cell Death Dis, 2018-05-01;9(5):499.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor
    Authors: AT Holdbrooks, CM Britain, SL Bellis
    J. Biol. Chem., 2017-12-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage
    Authors: A Chakrabort, KA Dorsett, HQ Trummell, ES Yang, PG Oliver, JA Bonner, DJ Buchsbaum, SL Bellis
    J. Biol. Chem., 2017-11-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Bispecific T cell engager (BiTE�) antibody constructs can mediate bystander tumor cell killing
    Authors: SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson
    PLoS ONE, 2017-08-24;12(8):e0183390.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. TNF? promotes CAR-dependent migration of leukocytes across epithelial monolayers
    Sci Rep, 2016-05-19;6(0):26321.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy.
    Authors: Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y
    PLoS ONE, 2015-04-10;10(4):e0122212.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Negative selection by apoptosis enriches progenitors in naive and expanded human umbilical cord blood grafts.
    Authors: Mizrahi K, Ash S, Peled T, Yaniv I, Stein J, Askenasy N
    Bone Marrow Transplant, 2014-04-28;49(7):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Apoptotic signaling through Fas and TNF receptors ameliorates GVHD in mobilized peripheral blood grafts.
    Authors: Mizrahi K, Yaniv I, Ash S, Stein J, Askenasy N
    Bone Marrow Transplant, 2014-02-24;49(5):640-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. High efficiency cell-specific targeting of cytokine activity.
    Authors: Garcin G, Paul F, Staufenbiel M, Bordat Y, Van der Heyden J, Wilmes S, Cartron G, Apparailly F, De Koker S, Piehler J, Tavernier J, Uze G
    Nat Commun, 2014-01-01;5(0):3016.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Tumor necrosis factor stimulates osteoclastogenesis from human bone marrow cells under hypoxic conditions.
    Authors: Nomura T, Aoyama M, Waguri-Nagaya Y, Goto Y, Suzuki M, Miyazawa K, Asai K, Goto S
    Exp Cell Res, 2013-12-17;321(2):167-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Enhancement of TWIK-related acid-sensitive potassium channel 3 (TASK3) two-pore domain potassium channel activity by tumor necrosis factor alpha.
    Authors: El Hachmane M, Rees K, Veale E, Sumbayev V, Mathie A
    J Biol Chem, 2013-12-04;289(3):1388-401.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. The transmembrane domains of TNF-related apoptosis-inducing ligand (TRAIL) receptors 1 and 2 co-regulate apoptotic signaling capacity.
    Authors: Neumann S, Bidon T, Branschadel M, Krippner-Heidenreich A, Scheurich P, Doszczak M
    PLoS ONE, 2012-08-03;7(8):e42526.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
    Authors: Renaud SJ, Sullivan R, Graham CH
    Placenta, 2009-02-08;30(4):313-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
    Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
    J. Allergy Clin. Immunol., 2008-12-05;123(1):239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Differential responses of FLIPLong and FLIPShort-overexpressing human myeloid leukemia cells to TNF-alpha and TRAIL-initiated apoptotic signals.
    Authors: Seal S, Hockenbery DM, Spaulding EY, Kiem HP, Abbassi N, Deeg HJ
    Exp. Hematol., 2008-10-05;36(12):1660-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
    Authors: Kolliputi N, Waxman AB
    Am. J. Respir. Cell Mol. Biol., 2008-09-05;40(3):314-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Death receptor expression is associated with poor response to chemotherapy and shorter survival in metastatic ovarian carcinoma.
    Authors: Dong HP, Kleinberg L, Silins I, Florenes VA, Trope CG, Risberg B, Nesland JM, Davidson B
    Cancer, 2008-01-01;112(1):84-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  27. A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
    Authors: Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK
    Cell Death Differ., 2006-01-20;13(10):1789-801.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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