Human TNF RI/TNFRSF1A APC-conjugated Antibody

Catalog #: FAB225A
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB225A

Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry.

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Citations (5)

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Human TNF RI/TNFRSF1A APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TNF RI in Western blots. In Western blots, does not cross-react with recombinant human TNF RII, recombinant mouse (rm) TNF RI, or rmTNF RII.

Source

Monoclonal Mouse IgG₁ Clone # 16803

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human TNF RI/TNFRSF1A
Accession # NP_001056

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TNF RI/TNFRSF1A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF RI/TNFRSF1A APC-conjugated Monoclonal Antibody (Catalog # FAB225A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF RI/TNFRSF1A

TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

Long Name

Tumor Necrosis Factor Receptor I

Entrez Gene IDs

7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)

Alternate Names

CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

Product Datasheets

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Citations for Human TNF RI/TNFRSF1A APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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The Influence of Severity and Disease Duration on TNF Receptors' Redistribution in Asthma and Rheumatoid Arthritis
Authors: A Alshevskay, J Lopatnikov, J Zhukova, O Chumasova, N Shkaruba, A Sizikov, I Evsegneeva, D Demina, V Nepomniash, A Karaulov, S Sennikov
Cells, 2022-12-20;12(1):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
TNF-alpha Affects Signature Cytokines of Th1 and Th17 T Cell Subsets through Differential Actions on TNFR1 and TNFR2
Authors: B Pesce, CH Ribeiro, M Larrondo, V Ramos, L Soto, D Catalán, JC Aguillón
International Journal of Molecular Sciences, 2022-08-18;23(16):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Co-expression of TNF receptors 1 and 2 on melanomas facilitates soluble TNF-induced resistance to MAPK pathway inhibitors
Authors: CA Sander, EA Rush, J Shi, LMRB Arantes, RJ Tesi, MA Ross, MJ Calderon, SC Watkins, JM Kirkwood, RL Ferris, LH Butterfiel, L Vujanovic
Journal of Translational Medicine, 2022-07-25;20(1):331.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
An in-depth comparison of latent HIV-1 reactivation in multiple cell model systems and resting CD4+ T cells from aviremic patients.
Authors: Spina, Celsa A, Anderson, Jenny, Archin, Nancie M, Bosque, Alberto, Chan, Jonathan, Famiglietti, Marylind, Greene, Warner C, Kashuba, Angela, Lewin, Sharon R, Margolis, David M, Mau, Matthew, Ruelas, Debbie, Saleh, Suha, Shirakawa, Kotaro, Siliciano, Robert F, Singhania, Akul, Soto, Paula C, Terry, Valeri H, Verdin, Eric, Woelk, Christop, Wooden, Stacey, Xing, Sifei, Planelles, Vicente
PLoS Pathog, 2013-12-26;9(12):e1003834.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Differential expression of TNFR1 (CD120a) and TNFR2 (CD120b) on subpopulations of human monocytes.
Authors: Hijdra D, Vorselaars A, Grutters J, Claessen A, Rijkers G
J Inflamm (Lond), 2012-10-05;9(1):38.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry