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Human TNF RII/TNFRSF1B Antibody

Catalog #: MAB726
11 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB726-SP
MAB726-100
MAB726-500
TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody.
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Citations (11)
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Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TNF RII/TNFRSF1B Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF RII in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) TNF RI, recombinant mouse (rm) TNF RI, rmTNF RII, rmTNF‑ alpha, rhTNF‑ alpha, or rhTNF‑ beta. In Western blots, this antibody does not
cross‑react with rhTNF RI, rmTNF RI, or rmTNF RII.
Source
Monoclonal Mouse IgG1 Clone # 22210
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TNF RII/TNFRSF1B
Leu23-Asp257
Accession # P20333
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2)
under non-reducing conditions only

Human TNF RII/TNFRSF1B Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human TNF RII/TNFRSF1B Biotinylated Antibody (Catalog # BAF726)

Standard: Recombinant Human TNF RII/TNFRSF1B (aa 24-206) Protein (Catalog # 1089-R2)

Neutralization
Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 30.0-300 ng/mL in the presence of 0.3 µg/mL Recombinant Human TNF RII/TNFRSF1B Fc Chimera, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization View Larger

TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody. Recombinant Human TNF RII/TNFRSF1B Fc Chimera (726-R2) inhibits Recombinant Human TNF-a (210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of Recombinant Human TNF-a (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/TNFRSF1B Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB726). The ND50 is typically 30.0-300 ng/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RII/TNFRSF1B

TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with high affinity and is expressed primarily in cells of the immune system.

Long Name
Tumor Necrosis Factor Receptor II
Entrez Gene IDs
7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)
Alternate Names
CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II

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Citations for Human TNF RII/TNFRSF1B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. TNF-alpha Affects Signature Cytokines of Th1 and Th17 T Cell Subsets through Differential Actions on TNFR1 and TNFR2
    Authors: B Pesce, CH Ribeiro, M Larrondo, V Ramos, L Soto, D Catalán, JC Aguillón
    International Journal of Molecular Sciences, 2022-08-18;23(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Inflammation-Driven Regulation of PD-L1 and PD-L2, and Their Cross-Interactions with Protective Soluble TNFalpha Receptors in Human Triple-Negative Breast Cancer
    Authors: T Baram, N Oren, N Erlichman, T Meshel, A Ben-Baruch
    Cancers, 2022-07-19;14(14):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1
    Authors: J Sellau, M Groneberg, H Fehling, T Thye, S Hoenow, C Marggraff, M Weskamm, C Hansen, S Stanelle-B, S Kuehl, J Noll, V Wolf, NG Metwally, SH Hagen, C Dorn, J Wernecke, H Ittrich, E Tannich, T Jacobs, I Bruchhaus, M Altfeld, H Lotter
    Nat Commun, 2020-07-10;11(1):3459.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  4. High Glucose Exacerbates TNF-&alpha-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1
    Authors: W Zhu, Q Qiu, H Luo, F Zhang, J Wu, X Zhu, M Liang
    Stem Cells Int, 2020-02-05;2020(0):4910767.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis
    Authors: Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su
    Oncogene, 2019-10-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Targeted killing of TNFR2-expressing tumor cells and Tregs by TNFR2 antagonistic antibodies in advanced S�zary syndrome
    Authors: H Torrey, M Khodadoust, L Tran, D Baum, A Defusco, YH Kim, DL Faustman
    Leukemia, 2018-10-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009-03-01;182(0):3112-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
    Authors: Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U
    J. Immunol., 2007-09-15;179(6):4239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004-01-01;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  10. Innate immunity associated with inflammatory responses and cytokine production against common dietary proteins in patients with autism spectrum disorder.
    Authors: Jyonouchi H, Sun S, Itokazu N
    Neuropsychobiology, 2002-01-01;46(2):76-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  11. A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis.
    Authors: Bharucha AE, 2019, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD
    2000-09-01;95(9):2338-42.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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Reviews for Human TNF RII/TNFRSF1B Antibody

Average Rating: 5 (Based on 3 Reviews)

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Human TNF RII/TNFRSF1B Antibody
By Anonymous on 08/28/2021
Application: WB Sample Tested: Serum Species: Human
Western Blot Human TNF RII/TNFRSF1B Antibody MAB726
Human TNF RII/TNFRSF1B Antibody
By Anonymous on 12/15/2017
Application: B/N Sample Tested: Cell culture supernatant Species: Human
Human TNF RII/TNFRSF1B Antibody
By Anonymous on 11/22/2017
Application: ELISA Sample Tested: Cell culture supernatant,Serum and Plasma Species: Human

We used MAB726 as the solid phase antibody, 1089-R2 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.

ELISA Human TNF RII/TNFRSF1B Antibody MAB726