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Human Total MMP-7 Quantikine ELISA Kit

Catalog #: DMP700
35 Citations Datasheet / COA / SDS
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PDMP700
SMP700
DMP700
Control Products Available
QC128: Quantikine Immunoassay Control Set 1180 Human Total MMP-7
Human Total MMP-7 ELISA Standard Curve
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Product Details
Procedure
Citations (35)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Total MMP-7 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (25 uL), Heparin Plasma (25 uL), Saliva (25 uL), Urine (50 uL)
Sensitivity
0.094 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human MMP-7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MMP-7 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MMP-7 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human MMP-7 and antibodies raised against the recombinant factor. The Quantikine kit detected both pro- and active forms of recombinant human MMP-7. Natural human MMP-7 showed dose-response curves that were parallel to the standard curves obtained using the kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-7.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 1.26 4.58 6.29 1.23 4.82 6.76
Standard Deviation 0.064 0.168 0.217 0.054 0.198 0.309
CV% 5.1 3.7 3.4 4.4 4.1 4.6

Recovery

The recovery of MMP-7 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 96-108
Urine (n=2) 99 89-109

Linearity

To assess the linearity of the assay, samples spiked with or containing high concentrations of MMP-7 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Human Total MMP-7 ELISA Linearity

Scientific Data

N/A Human Total MMP-7 ELISA Standard Curve View Larger

Human Total MMP-7 ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-7

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 7
Entrez Gene IDs:
4316 (Human); 17393 (Mouse)
Alternate Names:
EC 3.4.24; EC 3.4.24.23; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Total MMP-7 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Expression of Matrix Metalloproteinase-2,-7,-9 in Serum during Pregnancy in Patients with Pre-Eclampsia: A Prospective Study
    Authors: A Bahabayi, N Yang, T Xu, Y Xue, L Ma, X Gu, Y Wang, K Jia
    International Journal of Environmental Research and Public Health, 2022-11-04;19(21):.
    Species: Human
    Sample Types: Serum
  2. High Pretreatment Serum PD-L1 Levels Are Associated with Muscle Invasion and Shorter Survival in Upper Tract Urothelial Carcinoma
    Authors: Á Széles, PT Kovács, A Csizmarik, M Váradi, P Riesz, T Fazekas, S Váncsa, P Hegyi, C Oláh, S Tschirdewa, C Darr, U Krafft, V Grünwald, B Hadaschik, O Horváth, P Nyirády, T Szarvas
    Biomedicines, 2022-10-13;10(10):.
    Species: Human
    Sample Types: Serum
  3. Thymidylate Synthase Overexpression Drives the Invasive Phenotype in Colon Cancer Cells
    Authors: WM Ciszewski, M Chmielewsk, LA Wozniak, K Sobierajsk
    Oncogene, 2022-05-29;10(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Forkhead Box q1 promotes invasion and metastasis in colorectal cancer by activating the epidermal growth factor receptor pathway
    Authors: JJ Zhang, CX Cao, LL Wan, W Zhang, ZJ Liu, JL Wang, Q Guo, H Tang
    World Journal of Gastroenterology, 2022-05-07;28(17):1781-1797.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Elevated Pre-Treatment Serum MMP-7 Levels Are Associated with the Presence of Metastasis and Poor Survival in Upper Tract Urothelial Carcinoma
    Authors: PT Kovács, T Mayer, A Csizmarik, M Váradi, C Oláh, Á Széles, S Tschirdewa, U Krafft, B Hadaschik, P Nyirády, P Riesz, T Szarvas
    Biomedicines, 2022-03-17;10(3):.
    Species: Human
    Sample Types: Serum
  6. Circulating Levels of PD-L1, TIM-3 and MMP-7 Are Promising Biomarkers to Differentiate COVID-19 Patients That Require Invasive Mechanical Ventilation
    Authors: L Chavez-Gal, A Ruiz, K Martinez-E, H Aguilar-Du, M Torres, R Falfan-Val, G Pérez-Rubi, M Selman, I Buendia-Ro
    Biomolecules, 2022-03-14;12(3):.
    Species: Human
    Sample Types: Serum
  7. Differential Modulation of Matrix Metalloproteinases-2 and -7 in LAM/TSC Cells
    Authors: S Ancona, E Orpianesi, C Bernardell, E Chiaramont, R Chiaramont, S Terraneo, F Di Marco, E Lesma
    Biomedicines, 2021-11-24;9(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Cleavage of the Perlecan-Semaphorin 3A-Plexin A1-Neuropilin-1 (PSPN) Complex by Matrix Metalloproteinase 7/Matrilysin Triggers Prostate Cancer Cell Dyscohesion and Migration
    Authors: TV Tellman, LA Cruz, BJ Grindel, MC Farach-Car
    International Journal of Molecular Sciences, 2021-03-22;22(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Urinary Matrix Metalloproteinase-7 and Prediction of AKI Progression Post Cardiac Surgery
    Authors: F Fang, W Luo, M Yang, P Yang, X Yang
    Dis. Markers, 2019-11-19;2019(0):9217571.
    Species: Human
    Sample Types: Urine
  10. Lower levels of α-Klotho in serum are associated with decreased lung function in individuals with interstitial lung abnormalities
    Authors: I Buendia-Ro, N Machuca, M Mejía, M Maldonado, A Pardo, M Selman
    Sci Rep, 2019-07-25;9(1):10801.
    Species: Human
    Sample Types: Serum
  11. Senolytics in idiopathic pulmonary fibrosis: Results from a first-in-human, open-label, pilot study
    Authors: JN Justice, AM Nambiar, T Tchkonia, NK LeBrasseur, R Pascual, SK Hashmi, L Prata, MM Masternak, SB Kritchevsk, N Musi, JL Kirkland
    EBioMedicine, 2019-01-05;0(0):.
    Species: Human
    Sample Types: Serum
  12. Serum Matrix Metalloproteinase-7 Level is Associated with Fibrosis and Renal Survival in Patients with IgA Nephropathy
    Authors: J Zhang, P Ren, Y Wang, S Feng, C Wang, X Shen, C Weng, X Lang, Z Chen, H Jiang, J Chen
    Kidney Blood Press. Res., 2017-09-18;42(3):541-552.
    Species: Human
    Sample Types: Serum
  13. The interdependencies of viral load, the innate immune response, and clinical outcome in children presenting to the emergency department with respiratory syncytial virus-associated bronchiolitis
    Authors: FA Piedra, M Mei, V Avadhanula, R Mehta, L Aideyan, RP Garofalo, PA Piedra
    PLoS ONE, 2017-03-07;12(3):e0172953.
    Species: Human
    Sample Types: Nasal Lavage Fluid
  14. Mucin 4 and matrix metalloproteinase 7 as novel salivary biomarkers for periodontitis
    Authors: Anna Lundmark
    J. Clin. Periodontol, 2017-02-06;0(0):.
    Species: Human
    Sample Types: Saliva
  15. Concomitant elevations of MMP-9, NGAL, proMMP-9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease
    J. Cell. Mol. Med, 2016-12-22;0(0):.
    Species: Human
    Sample Types: Serum
  16. Suppression of Aurora-A-FLJ10540 signaling axis prohibits the malignant state of head and neck cancer.
    Authors: Chen, Chang-Ha, Chang, Alice Y, Li, Shau-Hsu, Tsai, Hsin-Tin, Shiu, Li-Yen, Su, Li-Jen, Wang, Wen-Lung, Chiu, Tai-Jen, Luo, Sheng-De, Huang, Tai-Lin, Chien, Chih-Yen
    Mol Cancer, 2015-04-12;14(0):83.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Role of SIRT1 in regulation of epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis.
    Authors: Chen I, Chiang W, Huang H, Chen P, Shen Y, Chiang H
    Mol Cancer, 2014-11-26;13(0):254.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival.
    Authors: Keeratichamroen S, Leelawat K, Thongtawee T
    Int. J. Oncol., 2011-06-17;39(4):873-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Stat3 and MMP7 contribute to pancreatic ductal adenocarcinoma initiation and progression.
    Authors: Fukuda A, Wang SC, Morris JP, Folias AE, Liou A, Kim GE, Akira S, Boucher KM, Firpo MA, Mulvihill SJ, Hebrok M
    Cancer Cell, 2011-04-12;19(4):441-55.
    Species: Human
    Sample Types: Serum
  20. Elevated serum matrix metalloproteinase 7 levels predict poor prognosis after radical prostatectomy.
    Authors: Szarvas T, Becker M, vom Dorp F, Meschede J, Scherag A, Bankfalvi A, Reis H, Schmid KW, Romics I, Rubben H, Ergun S
    Int. J. Cancer, 2011-03-15;128(6):1486-92.
    Species: Human
    Sample Types: Serum
  21. Validation of circulating MMP-7 level as an independent prognostic marker of poor survival in urinary bladder cancer.
    Authors: Szarvas T, Jager T, Becker M, Tschirdewahn S, Niedworok C, Kovalszky I, Rubben H, Ergun S, vom Dorp F
    Pathol. Oncol. Res., 2011-01-21;17(2):325-32.
    Species: Human
    Sample Types: Plasma
  22. Urinary matrix metalloproteinase-7 level is associated with the presence of metastasis in bladder cancer.
    Authors: Szarvas T, Singer BB, Becker M, vom Dorp F, Jager T, Szendroi A, Riesz P, Romics I, Rubben H, Ergun S
    BJU Int., 2010-09-03;107(7):1069-73.
    Species: Human
    Sample Types: Urine
  23. Matrix metalloproteinase-7 as a marker of metastasis and predictor of poor survival in bladder cancer.
    Authors: Szarvas T, Becker M, vom Dorp F, Gethmann C, Totsch M, Bankfalvi A, Schmid KW, Romics I, Rubben H, Ergun S
    Cancer Sci., 2010-01-20;101(5):1300-8.
    Species: Human
    Sample Types: Serum
  24. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2009-11-17;84(3):239-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Elevated serum matrix metalloproteinase-3 and -7 in H. pylori-related gastric cancer can be biomarkers correlating with a poor survival.
    Authors: Yeh YC, Sheu BS, Cheng HC, Wang YL, Yang HB, Wu JJ
    Dig. Dis. Sci., 2009-08-19;55(6):1649-57.
    Species: Human
    Sample Types: Serum
  26. Fibulin-5 suppresses lung cancer invasion by inhibiting matrix metalloproteinase-7 expression.
    Authors: Yue W, Sun Q, Landreneau R, Wu C, Siegfried JM, Yu J, Zhang L
    Cancer Res., 2009-07-07;69(15):6339-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Gingival fibroblast inhibits MMP-7: evaluation in an ex vivo aorta model.
    Authors: Gogly B, Fournier B, Couty L, Naveau A, Brasselet C, Durand E, Coulomb B, Lafont A
    J. Mol. Cell. Cardiol., 2009-05-03;47(2):296-303.
    Species: Rabbit
    Sample Types: Cell Culture Supernates
  28. Circulating matrix metalloproteinase-7: an early or metastatic marker for renal cell carcinoma?
    Authors: Jung K, Ramankulov A, Schrader M, Miller K, Lein M
    Clin. Chem., 2008-11-01;54(11):1927-9.
    Species: Human
    Sample Types: Plasma
  29. MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathic pulmonary fibrosis.
    Authors: Rosas IO, Richards TJ, Konishi K, Zhang Y, Gibson K, Lokshin AE, Lindell KO, Cisneros J, Macdonald SD, Pardo A, Sciurba F, Dauber J, Selman M, Gochuico BR, Kaminski N
    PLoS Med., 2008-04-29;5(4):e93.
    Species: Human
    Sample Types: Serum
  30. Is metalloproteinase-7 specific for idiopathic pulmonary fibrosis?
    Authors: Huh JW, Kim DS, Oh YM, Shim TS, Lim CM, Lee SD, Koh Y, Kim WS, Kim WD, Kim KR
    Chest, 2007-12-10;133(5):1101-6.
    Species: Human
    Sample Types: BALF
  31. Determination of matrilysin activity in gastrointestinal neoplasia.
    Authors: Hawinkels LJ, Verspaget HW, van den Berg M, Hanemaaijer R, Sier CF
    Eur. J. Clin. Invest., 2007-07-01;37(7):598-9.
    Species: Human
    Sample Types: Tissue Homogenates
  32. Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy?
    Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE
    Kidney Int., 2007-06-06;72(4):481-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Generation of endostatin by matrix metalloproteinase and cathepsin from human limbocorneal epithelial cells cultivated on amniotic membrane.
    Authors: Ma DH, Yao JY, Kuo MT, See LC, Lin KY, Chen SC, Chen JK, Chao AS, Wang SF, Lin KK
    Invest. Ophthalmol. Vis. Sci., 2007-02-01;48(2):644-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. An integrated clinical-genomics approach identifies a candidate multi-analyte blood test for serous ovarian carcinoma.
    Authors: Meinhold-Heerlein I, Bauerschlag D, Zhou Y, Sapinoso LM, Ching K, Frierson H, Brautigam K, Sehouli J, Stickeler E, Konsgen D, Hilpert F, von Kaisenberg CS, Pfisterer J, Bauknecht T, Jonat W, Arnold N, Hampton GM
    Clin. Cancer Res., 2007-01-15;13(2):458-66.
    Species: Human
    Sample Types: Serum
  35. Serum biobank certification and the establishment of quality controls for biological fluids: examples of serum biomarker stability after temperature variation.
    Authors: Chaigneau C, Cabioch T, Beaumont K, Betsou F
    Clin. Chem. Lab. Med., 2007-01-01;45(10):1390-5.
    Species: Human
    Sample Types: Serum

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