Human TRAF-2 Antibody

Catalog #: AF3277
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3277
AF3277-SP

Detection of Human TRAF‑2 by Western Blot.

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All TRAF-2 Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRAF-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRAF-2 in Western blots. In Western blots, less than 1% cross-reacivity with recombinant human TRAF-1, -3, -4, -5, or -6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TRAF-2
Met1-Leu501
Accession # Q12933

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Knockout Validated

TRAF‑2 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in TRAF‑2 knockout HEK293T cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TRAF‑2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human TRAF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-2 was detected at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Knockout Validated

Western Blot Shows Human TRAF-2 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human TRAF‑2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TRAF-2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human TRAF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TRAF-2 at approximately 62 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-2

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-2, also known as TNF Receptor-Associated Protein (TRAP), is a 501 amino acid, 56 kDa protein which interacts with the cytoplasmic domain of TNFR1, TNFR2 and CD40 to mediate the activation of NF-kappa-B and AP-1 family of transcription factors. TRAF-2 can self-associate as well as form heterodimers with TRAF-1.

Long Name

TNF Receptor-Associated Factor 2

Entrez Gene IDs

7186 (Human); 22030 (Mouse)

Alternate Names

E3 ubiquitin-protein ligase TRAF2; EC 6.3.2.-; MGC:45012; TNF receptor-associated factor 2; TRAF2; TRAF-2; TRAP3; TRAP3TRAP; tumor necrosis factor type 2 receptor associated protein 3; Tumor necrosis factor type 2 receptor-associated protein 3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human TRAF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells
Authors: Y Shlyakhtin, V Pavet, H Gronemeyer
Cell Death Dis, 2017-08-31;8(8):e3025.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation