Human TrkA Antibody

Catalog #: AF175
10 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF175
AF175-SP

Detection of Human TrkA by Western Blot.

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All TrkA Antibodies

Product Details

Citations (10)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TrkA Antibody Summary

Species Reactivity

Human

Specificity

Detects human TrkA in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat TrkA is observed and 10% cross-reactivity with recombinant human (rh) TrkB and rhTrKC is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TrkA
Ala33-Glu407
Accession # AAA36770

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

K562 human chronic myelogenous leukemia cell line

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 3-12 µg/mL in the presence of 5 ng/mL Recombinant Human beta ‑NGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TrkA by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF175) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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TrkA in Human Colon. TrkA was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF175) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in colon glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization

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Cell Proliferation Induced by beta ‑NGF and Neutralization by Human TrkA Antibody. Recombinant Human beta -NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta -NGF (5 ng/mL) is neutralized (green line) by increasing concent-rations of Goat Anti-Human TrkA Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF175). The ND₅₀ is typically 3-12 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily. NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when expressed independent of Trk.

References

Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat)

Alternate Names

DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TrkA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons
Authors: AJ Hulme, JR McArthur, S Maksour, S Miellet, L Ooi, DJ Adams, RK Finol-Urda, M Dottori
Frontiers in Cellular Neuroscience, 2020-12-04;14(0):600895.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Nerve growth factor receptor role on rabbit sperm storage
Authors: C Castellini, S Mattioli, AD Bosco, E Cotozzolo, A Cartoni Ma, M Rende, AM Stabile, A Pistilli
Theriogenology, 2020-05-11;153(0):54-61.
Species: Rabbit
Sample Types: Whole Cells, Whole Cells
Applications: Flow Cytometry, Neutralization
In vitro effect of nerve growth factor on the main traits of rabbit sperm
Authors: C Castellini, S Mattioli, A Dal Bosco, G Collodel, A Pistilli, AM Stabile, L Macchioni, F Mancuso, G Luca, M Rende
Reprod. Biol. Endocrinol., 2019-11-12;17(1):93.
Species: Rabbit
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Neutralization, Western Blot
Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA
Authors: S Desiderio, S Vermeiren, C Van Campen, S Kricha, E Malki, S Richts, EV Fletcher, T Vanwelden, BZ Schmidt, KA Henningfel, T Pieler, CG Woods, V Nagy, C Verfaillie, EJ Bellefroid
Cell Rep, 2019-03-26;26(13):3522-3536.e5.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
Authors: AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
Sci Rep, 2018-01-12;8(1):603.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Nerve growth factor downregulates inflammatory response in human monocytes through TrkA.
Authors: Prencipe G, Minnone G, Strippoli R, De Pasquale L, Petrini S, Caiello I, Manni L, De Benedetti F, Bracci-Laudiero L
J Immunol, 2014-02-28;192(7):3345-54.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection.
Authors: Flowerdew S, Wick D, Himmelein S, Horn A, Sinicina I, Strupp M, Brandt T, Theil D, Hufner K
PLoS ONE, 2013-12-19;8(12):e83603.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
TrkB antibody elicits cytotoxicity and suppresses migration/invasion of transitional cell carcinoma cells.
Authors: Huang YT, Lai PC, Wu CC
Int. J. Oncol., 2010-10-01;37(4):943-9.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells.
Authors: Huang YT, Lai PC, Wu CC, Hsu SH, Cheng CC, Lan YF, Chiu TH
Int. J. Oncol., 2010-06-01;36(6):1469-76.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Neurotrophin signaling through tropomyosin receptor kinases contributes to survival and proliferation of non-Hodgkin lymphoma.
Authors: Sniderhan LF, Garcia-Bates TM, Burgart M, Bernstein SH, Phipps RP, Maggirwar SB
Exp. Hematol., 2009-08-28;37(11):1295-309.
Species: Human
Sample Types: Whole Cells
Applications: ICC