Human TROP-2 APC-conjugated Antibody

Catalog #: FAB650A
3 Citations Datasheet / COA / SDS

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FAB650A

Detection of TROP-2 in PC-3 Human Cell Line by Flow Cytometry.

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All TROP-2 Antibodies

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TROP-2 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) VCAM‑1 or rhICAM‑1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 77220

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TROP-2
His27-Thr274
Accession # P09758

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TROP-2 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TROP-2 APC-conjugated Monoclonal Antibody (Catalog # FAB650A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

References

Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
Ripani, E. et al. (1998) Int. J. Cancer 76:671.
El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Long Name

Tumor-associated Calcium Signal Transducer 2

Entrez Gene IDs

4070 (Human); 56753 (Mouse)

Alternate Names

Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human TROP-2 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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TACSTD2 upregulation is an early reaction to lung infection
Authors: S Lenárt, P Lenárt, L Knopfová, H Kotasová, V Pelková, V Sedláková, O Vacek, J Pokludová, V ?an, J Šmarda, K Sou?ek, A Hampl, P Beneš
Oncogene, 2022-06-10;12(1):9583.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Prostate epithelial cell of origin determines cancer differentiation state in an organoid transformation assay
Authors: JW Park, JK Lee, JW Phillips, P Huang, D Cheng, J Huang, ON Witte
Proc Natl Acad Sci USA, 2016-04-04;113(16):4482-7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Estrogen Receptor beta Activation Impairs Prostatic Regeneration by Inducing Apoptosis in Murine and Human Stem/Progenitor Enriched Cell Populations.
Authors: Hussain S, Lawrence MG, Taylor RA, Lo CY, Bioresource AP, Frydenberg M, Ellem SJ, Furic L, Risbridger GP
PLoS ONE, 2012-07-10;7(7):e40732.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry