Human TROP-2 Biotinylated Antibody

Catalog #: BAF650
2 Citations Datasheet / COA / SDS

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BAF650

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Citations (2)

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Human TROP-2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TROP-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human E‑Cadherin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TROP-2
Thr88-Thr274
Accession # P09758

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TROP‑2 Fc Chimera (Catalog # 650-T2)

Immunohistochemistry

3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TROP-2 in Human Cervical Cancer Tissue. TROP-2 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Goat Anti-Human TROP-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF650) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

References

Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
Ripani, E. et al. (1998) Int. J. Cancer 76:671.
El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.

Long Name

Tumor-associated Calcium Signal Transducer 2

Entrez Gene IDs

4070 (Human); 56753 (Mouse)

Alternate Names

Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

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Product Datasheet

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Related Research Areas

Citations for Human TROP-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers
Authors: H Lee, M Choi, J Lim, M Jo, JY Han, TM Kim, Y Cho
Theranostics, 2018-01-01;8(2):505-517.
Applications: Functional Assay
Trop2 identifies a subpopulation of murine and human prostate basal cells with stem cell characteristics.
Authors: Goldstein AS, Lawson DA, Cheng D, Sun W, Garraway IP, Witte ON
Proc. Natl. Acad. Sci. U.S.A., 2008-12-16;105(52):20882-7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry