Human Troponin T (Cardiac) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Troponin T in BG01V Human Embryonic Stem Cells. Troponin T was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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Troponin T in Human Heart. Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Troponin T
Troponin T is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury.
Product Datasheets
Citations for Human Troponin T (Cardiac) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Canonical Wnt signaling directs the generation of functional human PSC-derived atrioventricular canal cardiomyocytes in bioprinted cardiac tissues
Authors: Ye, C;Yang, C;Zhang, H;Gao, R;Liao, Y;Zhang, Y;Jie, L;Zhang, Y;Cheng, T;Wang, Y;Ren, J;
Cell stem cell
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Immunocytochemistry -
A combined human gastruloid model of cardiogenesis and neurogenesis
Authors: Olmsted ZT, Paluh JL.
iScience
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Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1
Authors: YH Chen, PS Huang, MH Wen, M Pan, DF Lee, TY Chen
Oncogene, 2022-06-14;63(0):102845.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
hESC derived cardiomyocyte biosensor to detect the different types of arrhythmogenic properties of drugs
Authors: R Pivato, S Klimovic, D Kabanov, F Sverák, M Pesl, J Pribyl, V Rotrekl
Oncogene, 2022-05-20;1216(0):339959.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
Authors: PS Huang, MH Wen, X Xie, A Xu, DF Lee, TY Chen
Stem Cell Research, 2021-06-06;54(0):102415.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease
Authors: J Jung, S Hwang, H Seol, AH Kim, HM Lee, JJ Sung, SM Jeong, YM Choi, JK Jun, DW Kim, J Jang
Stem Cell Research, 2021-04-10;53(0):102321.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Differentiation and characterization of rhesus monkey atrial and ventricular cardiomyocytes from induced pluripotent stem cells
Authors: X Zhang, H Cao, S Bai, W Huo, Y Ma
Stem Cell Res, 2017-02-11;20(0):21-29.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells
Authors: F Pei, J Jiang, S Bai, H Cao, L Tian, Y Zhao, C Yang, H Dong, Y Ma
Stem Cell Res, 2017-01-12;19(0):94-103.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals.
Authors: Zhang Q, Jiang J, Han P, Yuan Q, Zhang J, Zhang X, Xu Y, Cao H, Meng Q, Chen L, Tian T, Wang X, Li P, Hescheler J, Ji G, Ma Y
Cell Res., 2010-11-23;21(4):579-87.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
Cardiovasc. Res., 2010-07-29;89(1):157-65.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Stem cells derived from human fetal membranes display multilineage differentiation potential.
Authors: Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U
Biol. Reprod., 2007-05-09;77(3):577-88.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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We use this 'Human Troponin T (Cardiac) Antibody' for staining differentiated cardiac cells from non-muscle cells (iPSc, Fibroblasts, etc.).
