Human Ubiquitin+1 Antibody

Catalog # Availability Size / Price Qty
MAB7031
MAB7031-SP
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Human Ubiquitin+1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ubiquitin+1 in ELISAs and Western blots. In in ELISAs and Western blots, no cross-reactivity with Ubiquitin is observed.
Source
Monoclonal Mouse IgG2B Clone # 83456
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Ubiquitin+1
Met1-Gly75, YADLREDPDRQDHHPGSGAQ (C-terminal sequence generated by a frameshift)
Accession # CAA44911
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Ubiquitin+1 (Catalog # 703-UB)

Human Ubiquitin+1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Ubiquitin+1 Biotinylated Antibody (Catalog # BAM7032)

Standard: Recombinant Human Ubiquitin+1 Protein, CF (Catalog # 703-UB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ubiquitin+1

Ubiquitin+1 has a carboxyl terminal amino acid sequence that differs from normal Ubiquitin. The different carboxyl terminal sequence appears to result from a frameshift in the Ubiquitin mRNA. The underlying mechanisms creating the mRNA frameshift are not clearly understood. The occurrence of the frameshift that generates Ubiquitin+1 is much more prevalent in patients with Alzheimers Disease or with Down Syndrome than in control individuals who are not afflicted with the disorders. The monoclonal anti-Ubiquitin+1 (Catalog # MAB703) and rabbit polyclonal anti-Ubiquitin+1 (Catalog # AF703) antibodies were raised against the Ubiquitin+1 carboxyl terminal sequence that differs from normal Ubiquitin and are therefore non-reactive with Ubiquitin. Monoclonal anti-Ubiquitin (Catalog # MAB701) detects both Ubiquitin and Ubiquitin+1 indicating that the epitope recognized by this antibody is contained in the portion of the proteins that are identical (1, 2).

References
  1. van Leeuwen, F.W. et al. (1998) Science 279:242.
  2. van Leeuwen, F.W. et al. (1998) Trends Neurosci. 21:331.
Long Name
frame shift mutant
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
FLJ25987; MGC8385; polyubiquitin B; polyubiquitin-B; RPS27A; UBA52; UBC; ubiquitin B; Ubiquitin+1

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