Human UNC13D Alternative Isoform Antibody

Catalog #: MAB8966 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8966
MAB8966-SP

Detection of Human UNC13D by Western Blot.

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All UNC13D Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UNC13D Alternative Isoform Antibody Summary

Species Reactivity

Human

Specificity

Detects the alternate form of human UNC13D in Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1222B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

KLH-coupled N-terminal human UNC13D peptide of an alternative isoform described in PMID:24842371
Accession # EAW89322

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.05 µg/mL

See below

Simple Western

0.5 µg/mL

See below

Immunocytochemistry

2-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human UNC13D by Western Blot. Western blot shows lysates of NK-92 human natural killer lymphoma cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB8966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for UNC13D at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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UNC13D in NK‑92 Human Cell Line. UNC13D was detected in immersion fixed NK-92 human natural killer lymphoma cell line using Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB8966) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western

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Detection of Human UNC13D by Simple Western^TM. Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB8966). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UNC13D

UNC13D (also Munc13-4) is a 123 kDa cytoplasmic and peripheral membrane protein that is expressed at highest levels in hematopoietic tissues. UNC13D appears to play a role in vesicle maturation during exocytosis and its expression is obligatory for exocytosis of cytolytic granules from NK and T cells. A point mutation in intron 1 of UNC13D causes familial hematophagocytic lymphohistiocytosis type3, a rare autosomal recessive immune deficiency. The mutation disrupts transcription factor binding and prevents expression of an alternative isoform that is required for lymphocyte cytotoxicity. The conventional and alternative isoforms are identical from amino acid 40-1090 but have distinct N-terminal segments. It is unknown whether mice express the alternative isoform.

Long Name

UNC-13 Homolog D

Entrez Gene IDs

201294 (Human); 70450 (Mouse); 192177 (Rat)

Alternate Names

FHL3; HLH3; HPLH3; Munc13-4; Munc13-4protein unc-13 homolog D; unc-13 homolog D (C. elegans); UNC13D