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Human VAMP-7 Antibody

Catalog #: MAB6117
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6117
MAB6117-SP
Detection of Human VAMP‑7 by Western Blot.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VAMP-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VAMP‑7 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 549115
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human VAMP‑7
Ala2-Met140
Accession # P51809
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion-fixed paraffin-embedded sections of human breast and human breast cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human VAMP-7 antibody by Western Blot. View Larger

Detection of Human VAMP‑7 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human VAMP-7 Monoclonal Antibody (Catalog # MAB6117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAMP-7

Vesicle-associated membrane protein 7 (VAMP-7) is a 25 kDa, widely expressed, type IV transmembrane protein and member of the synaptobrevin family. Mature human VAMP-7 consists of a 187 aa cytoplasmic domain, a 21 aa transmembrane region, and an 11 aa vesicular region. The cytoplasmic domain contains a longin domain (aa 7‑110) and a v-SNARE coiled-coil homology domain (aa 125‑185). Two splicing variants produce three isoforms for human VAMP-7. Isoform 2 has a 116 aa substitution for aa 145‑220 found in isoform 1, and isoform 3 is missing the residues corresponding to aa 28‑68 in isoform 1. Human VAMP-7 shares 99%, 97%, and 95% aa sequence identity with bovine, mouse, and rat VAMP-7, respectively.

Long Name
Vesicle-Associated Membrane Protein 7
Entrez Gene IDs
6845 (Human); 20955 (Mouse); 85491 (Rat)
Alternate Names
FLJ54296; SYBL1; SYBL1FLJ53762; synaptobrevin-like 1; Synaptobrevin-like protein 1; tetanus neurotoxin-insensitive VAMP; Tetanus-insensitive VAMP; TIVAMP; TI-VAMP; VAMP7; VAMP-7; VAMP-7FLJ53045; vesicle-associated membrane protein 7

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Citations for Human VAMP-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Chylomicron production is repressed by RPTOR knockdown, R-alpha-lipoic acid and 4-phenylbutyric acid in human enterocyte-like Caco-2 cells
    Authors: B He, Z Wang, R Moreau
    The Journal of nutritional biochemistry, 2022-06-10;0(0):109087.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-?-stimulated lung epithelial cells
    Authors: YD Chen, YT Fang, YL Cheng, CF Lin, LJ Hsu, SY Wang, R Anderson, CP Chang, YS Lin
    Sci Rep, 2017-07-18;7(1):5676.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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