Human VAP-A Antibody Summary
Ala2-Met132
Accession # Q9P0L0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human VAP-A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human VAP-A Monoclonal Antibody (Catalog # MAB5820) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAP-A at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1with 0.05% Tween 20.
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VAP‑A in Human Brain. VAP-A was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human VAP-A Monoclonal Antibody (Catalog # MAB5820) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VAP-A
Vesicle-associated membrane protein (VAMP)-associated protein A (VAP-A; also VAMP-A and VAP-33) is a 33 kDa, ubiquitously expressed, type IV transmembrane protein belonging to the VAP family of proteins (1). It is found in plasma and ER membranes as well as in intracellular vesicles as a homodimer and a heterodimer with VAP-B. Human VAP-A is synthesized as a 249 amino acid (aa) precursor that contains a 227 aa cytoplasmic domain and a 21 aa transmembrane region. The cytoplasmic domain contains a mobile sperm protein (MSP) domain (aa 13-131) and a coiled-coil region (aa 169-205). Human VAP-A is 97% aa identical to mouse and rat VAP-A. VAP-A and VAP-B recruit FFAT (two phenylalanines in an acidic tract)-motif-containing proteins to the cytosolic surface of ER membranes through a conserved region within their MSP domain, and they have been implicated in regulation of membrane transport, phospholipid biosynthesis, and the unfolded protein response (2, 3). Their ability to interact with lipid-transfer/binding proteins (LT/BPs) may affect the lipid composition of certain cellular membranes (2, 4). VAPs play a critical role in maintaining the structural and functional properties of the Golgi complex (2). Knockdown of VAP reduces the levels of phosphatidylinositol‑4‑phosphate (PI4P), diacylglycerol (DAG), and sphingomyelin (SM) in Golgi membranes and exports pleiotropic effects in Golgi-mediated transport (2). The effects of VAPs are mediated by their interacting FFAT-motif-containing proteins Nir2, OSBP, and CERT (2). VAPs provide a scaffold for these LT/BPs at the ER-Golgi membrane contact sites, thereby affecting the lipid composition of the Golgi membranes and consequently their structural and functional identities (2). VAP-A associates with and regulates the neurite outgrowth-promoting activity of protrudin, a protein that promotes neurite formation (5).
- Weir, M.L. et al. (1998) Biochem. J. 333:247.
- Peretti, D. et al. (2008) Mol. Biol. Cell 19:3871.
- Kaiser, S.E. et al. (2005) Structure 13:1035.
- Loewen, C.J. et al. (2003) EMBO J. 22:2025.
- Saita, S. et al. (2009) J. Biol. Chem. 284:13766.
Product Datasheets
Citations for Human VAP-A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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NPC1 regulates ER contacts with endocytic organelles to mediate cholesterol egress
Authors: D Höglinger, T Burgoyne, E Sanchez-He, P Hartwig, A Colaco, J Newton, CE Futter, S Spiegel, FM Platt, ER Eden
Nat Commun, 2019-09-19;10(1):4276.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway
Authors: D Mao, G Lin, B Tepe, Z Zuo, KL Tan, M Senturk, S Zhang, BR Arenkiel, M Sardiello, HJ Bellen
Autophagy, 2019-02-20;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Kv2 potassium channels form endoplasmic reticulum/plasma membrane junctions via interaction with VAPA and VAPB
Authors: Ben Johnson, Ashley N. Leek, Laura Solé, Emily E. Maverick, Tim P. Levine, Michael M. Tamkun
Proceedings of the National Academy of Sciences
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