Human VE-Cadherin Antibody

Catalog #: MAB938
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB938
MAB938-SP

All VE-Cadherin Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VE-Cadherin Antibody Summary

Species Reactivity

Human

Specificity

Detects human VE-Cadherin in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) Cadherin-8, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, or rmVE-Cadherin.

Source

Monoclonal Mouse IgG₁ Clone # 123433

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human VE-Cadherin Fc Chimera (Catalog # 938-VC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.

References

Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
Overduin, M. et al. (1995) Science 267:386.
Takeichi, M. (1991) Science 251:1451.
Nose, A. et al. (1987) EMBO J. 6:3655.
Carmeliet, P. et al. (1999) Cell 98:147.
Gory-Faure, S. et al. (1999) Development 126:2093.

Long Name

Vascular Endothelium Cadherin

Entrez Gene IDs

1003 (Human); 12562 (Mouse)

Alternate Names

7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human VE-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Increased survivin expression confers chemoresistance to tumor-associated endothelial cells.
Authors: Virrey JJ, Guan S, Li W, Schonthal AH, Chen TC, Hofman FM
Am. J. Pathol., 2008-07-03;173(0):575-85.
Species: Human
Sample Types: Whole Cells
Applications: ICC