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Human VE-Cadherin Antibody

Catalog #: AF938
24 Citations 1 Review Datasheet / COA / SDS
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AF938
AF938-SP
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Detection of Human VE‑Cadherin by Western Blot.
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Citations (24)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VE-Cadherin Antibody Summary

Species Reactivity
Human
Specificity
Detects human VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse VE‑Cadherin is observed and less than 5% cross-reactivity with recombinant human E-Cadherin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
12.5 µg/mL
HUVEC human umbilical vein endothelial cells

Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human VE-Cadherin antibody by Western Blot. View Larger

Detection of Human VE‑Cadherin by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry VE-Cadherin antibody in HUVEC Cells by Immunocytochemistry (ICC). View Larger

VE‑Cadherin in HUVEC Cells. VE-Cadherin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western View Larger

Detection of Human VE‑Cadherin by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for VE‑Cadherin at approximately 162 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are
calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.

References
  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
  6. Carmeliet, P. et al. (1999) Cell 98:147.
  7. Gory-Faure, S. et al. (1999) Development 126:2093.
Long Name
Vascular Endothelium Cadherin
Entrez Gene IDs
1003 (Human); 12562 (Mouse)
Alternate Names
7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin

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Citations for Human VE-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. A novel efficient strategy to generate liver sinusoidal endothelial cells from human pluripotent stem cells
    Authors: Tian, SP;Ge, JY;Song, YM;Yu, XQ;Chen, WH;Chen, YY;Ye, D;Zheng, YW;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. ZO-1 interacts with YB-1 in endothelial cells to regulate stress granule formation during angiogenesis
    Authors: El Bakkouri, Y;Chidiac, R;Delisle, C;Corriveau, J;Cagnone, G;Gaonac'h-Lovejoy, V;Chin, A;Lécuyer, É;Angers, S;Joyal, JS;Topisirovic, I;Hulea, L;Dubrac, A;Gratton, JP;
    Nature communications
    Species: Bovine
    Sample Types: cell line
    Applications: Westen Blot, IF/ICC
  3. Regulation of angiogenesis by endocytic trafficking mediated by cytoplasmic dynein 1 light intermediate chain 1
    Authors: Johnson, D;Colijn, S;Richee, J;Yano, J;Burns, M;Davis, AE;Pham, VN;Saric, A;Jain, A;Yin, Y;Castranova, D;Melani, M;Fujita, M;Grainger, S;Bonifacino, JS;Weinstein, BM;Stratman, AN;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  4. CXCR3-CXCL11 signaling restricts angiogenesis and promotes pericyte recruitment
    Authors: Goeckel, ME;Lee, J;Levitas, A;Colijn, S;Mun, G;Burton, Z;Chintalapati, B;Yin, Y;Abello, J;Stratman, A;
    bioRxiv : the preprint server for biology
    Species: Fish - Danio rerio (Zebrafish)
    Sample Types: Whole Cells
    Applications: Immunocytochemistry/ Immunofluorescence
  5. Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against Inflammation
    Authors: Hong, SG;Ashby, JW;Kennelly, JP;Wu, M;Chattopadhyay, E;Foreman, R;Tontonoz, P;Turowski, P;Gallagher-Jones, M;Mack, JJ;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  6. Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis
    Authors: M Liu, S Samant, CH Vasa, RM Pedrigi, UM Oguz, S Ryu, T Wei, DR Anderson, DK Agrawal, YS Chatzizisi
    PLoS ONE, 2023-01-20;18(1):e0280385.
    Species: Human
    Sample Types: Whole, Whole Cells
    Applications: ICC
  7. NOTCH3 drives meningioma tumorigenesis and resistance to radiotherapy
    Authors: Choudhury, A;Cady, M;Lucas, C;Najem, H;Phillips, J;Palikuqi, B;Zakimi, N;Joseph, T;Birrueta, J;Chen, W;Bush, N;Hervey-Jumper, S;Klein, O;Toedebusch, C;Horbinski, C;Magill, S;Bhaduri, A;Perry, A;Dickinson, P;Heimberger, A;Ashworth, A;Crouch, E;Raleigh, D;
    bioRxiv
    Species: Human
    Sample Types: Whole Tissue
    Applications: RNAscope compatible
  8. Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin
    Authors: H Kim, K Leng, J Park, AG Sorets, S Kim, A Shostak, RJ Embalabala, K Mlouk, KA Katdare, IVL Rose, SM Sturgeon, EH Neal, Y Ao, S Wang, MV Sofroniew, JM Brunger, DG McMahon, MS Schrag, M Kampmann, ES Lippmann
    Nature Communications, 2022-11-02;13(1):6581.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Nascent polypeptide-Associated Complex and Signal Recognition Particle have cardiac-specific roles in heart development and remodeling
    Authors: AM Schroeder, T Nielsen, M Lynott, G Vogler, AR Colas, R Bodmer
    PloS Genetics, 2022-10-14;18(10):e1010448.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Murine endothelial serine palmitoyltransferase 1 (SPTLC1) is required for vascular development and systemic sphingolipid homeostasis
    Authors: Kuo A, Checa A, Niaudet C et al.
    eLife
  11. Peristaltic pumps adapted for laminar flow experiments enhance in vitro modeling of vascular cell behavior
    Authors: J Abello, S Raghavan, YY Yien, AN Stratman
    The Journal of Biological Chemistry, 2022-08-19;0(0):102404.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. LDLR expression in the cochlea suggests a role in endolymph homeostasis and cochlear amplification
    Authors: A Saume, M Thiry, J Defourny
    Hearing research, 2021-07-15;409(0):108311.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS
    Authors: YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao
    Aging Cell, 2021-06-04;0(0):e13388.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  14. Genetic disruption of the Blood Brain Barrier leads to protective barrier formation at the Glia Limitans
    Authors: P Mora, PL Hollier, S Guimbal, A Abelanet, A Diop, L Cornuault, T Couffinhal, S Horng, AP Gadeau, MA Renault, C Chapouly
    PLoS Biol, 2020-11-30;18(11):e3000946.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  15. Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment
    Authors: J Ohmes, Y Xiao, F Wang, MD Mikkelsen, TT Nguyen, H Schmidt, A Seekamp, AS Meyer, S Fuchs
    Mar Drugs, 2020-09-21;18(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes
    Authors: JW Blanchard, M Bula, J Davila-Vel, LA Akay, L Zhu, A Frank, MB Victor, JM Bonner, H Mathys, YT Lin, T Ko, DA Bennett, HP Cam, M Kellis, LH Tsai
    Nat. Med., 2020-06-08;26(6):952-963.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Identification of a combination of transcription factors that synergistically increases endothelial cell barrier resistance
    Authors: F Roudnicky, BK Kim, Y Lan, R Schmucki, V Küppers, K Christense, M Graf, C Patsch, M Burcin, CA Meyer, PD Westenskow, CA Cowan
    Sci Rep, 2020-03-03;10(1):3886.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability
    Authors: M Riddell, A Nakayama, T Hikita, F Mirzapours, T Kawamura, A Pasha, M Li, M Masuzawa, M Looso, T Steinbache, K Ebnet, M Potente, T Hirose, S Ohno, I Fleming, S Gattenlöhn, PP Aung, T Phung, O Yamasaki, T Yanagi, H Umemura, M Nakayama
    Nat Commun, 2018-12-17;9(1):5357.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation
    Authors: K Nonomura, V Lukacs, DT Sweet, LM Goddard, A Kanie, T Whitwam, SS Ranade, T Fujimori, ML Kahn, A Patapoutia
    Proc. Natl. Acad. Sci. U.S.A., 2018-11-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Vascular CXCR4 expression promotes vessel sprouting and sensitivity to sorafenib treatment in hepatocellular carcinoma
    Authors: J Xu, J Liang, YM Meng, J Yan, XJ Yu, CQ Liu, L Xu, SM Zhuang, L Zheng
    Clin. Cancer Res, 2017-02-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. DeepCAGE transcriptomics identify HOXD10 as transcription factor regulating lymphatic endothelial responses to VEGF-C
    J Cell Sci, 2016-05-19;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus.
    Authors: Wang J, Tao J, Chen D, Cai J, Irani K, Wang Q, Yuan H, Chen A
    Arterioscler Thromb Vasc Biol, 2013-10-31;34(0):99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
    Authors: Niwa A, Heike T, Umeda K
    PLoS ONE, 2011-07-27;6(7):e22261.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Signaling hierarchy regulating human endothelial cell development.
    Authors: Kelly MA, Hirschi KK
    Arterioscler. Thromb. Vasc. Biol., 2009-02-12;29(5):718-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human VE-Cadherin Antibody
By Anonymous on 06/09/2021
Application: ICC/IF Sample Tested: HMVEC human microvascular endothelial cells Species: Human
Immunocytochemistry/Immunofluorescence Human VE-Cadherin Antibody AF938