Human VEGF-C Antibody

Catalog #: MAB752
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB752-SP
MAB752-100
MAB752-500

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All VEGF-C Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGF-C Antibody Summary

Species Reactivity

Human

Specificity

Detects human VEGF-C in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) PDGF‑AA, rhPDGF‑BB, rhPlGF, rhVEGF₁₆₅, rhVEGF₂₀₆, rhVEGF-B₁₈₆, or rhVEGF-D.

Source

Monoclonal Mouse IgG_2B Clone # 193208

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VEGF-C
Thr103-Arg227 (Cys156Ser)
Accession # Q6FH59

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human VEGF-C (Catalog # 2179-VC)

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human VEGF-C DuoSet ELISA Kit (Catalog # DY752B) for convenient development of a sandwich ELISA or the Human VEGF-C Quantikine ELISA Kit (Catalog # DVEC00) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human VEGF‑C ELISA Standard Curve. Recombinant Human VEGF‑C protein was serially diluted 2-fold and captured by Mouse Anti-Human VEGF‑C Monoclonal Antibody (Catalog # MAB752) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (AF752) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-C

Vascular Endothelial Growth Factor C (VEGF-C) is a member of the VEGF family of secreted growth factors. The VHD form homodimers after proteolytic removal of the N- and C-terminal pro regions. VEGF-C promotes angiogenesis and lymphangiogenesis through interactions with VEGF R2 and VEGF R3.

Long Name

Vascular Endothelial Growth Factor C

Entrez Gene IDs

7424 (Human); 22341 (Mouse)

Alternate Names

Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human VEGF-C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1