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Human VEGFR2/KDR/Flk-1 Fluorescein-conjugated Antibody

Catalog #: FAB357F
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB357F-100
FAB357F-025
Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR2/KDR/Flk-1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 89106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1
Ala20-Glu764
Accession # P35968
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VEGF R2/KDR/Flk-1 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk-1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB357F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGFR2/KDR/Flk-1

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Human VEGFR2/KDR/Flk-1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Intravenous Administration of Allogenic Cell-Derived Microvesicles of Healthy Origins Defend Against Atherosclerotic Cardiovascular Disease Development by a Direct Action on Endothelial Progenitor Cells
    Authors: N Alexandru, E Andrei, F Safciuc, E Dragan, AM Balahura, E Badila, A Georgescu
    Cells, 2020-02-12;9(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Leucocyte and Platelet-rich Fibrin: a carrier of autologous multipotent cells for regenerative medicine
    Authors: R Di Liddo, T Bertalot, A Borean, I Pirola, A Argentoni, S Schrenk, C Cenzi, S Capelli, MT Conconi, PP Parnigotto
    J. Cell. Mol. Med., 2018-01-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Engineered ligand-based VEGFR antagonists with increased receptor binding affinity more effectively inhibit angiogenesis
    Authors: S Kapur, AP Silverman, AZ Ye, N Papo, D Jindal, MS Blumenkran, JR Cochran
    Bioeng Transl Med, 2017-02-17;2(1):81-91.
    Applications: Flow Cytometry
  4. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
    PLoS ONE, 2016-08-25;11(8):e0160153.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Levels of circulating endothelial cells are low in idiopathic pulmonary fibrosis and are further reduced by anti-fibrotic treatments.
    Authors: De Biasi S, Cerri S, Bianchini E, Gibellini L, Persiani E, Montanari G, Luppi F, Carbonelli C, Zucchi L, Bocchino M, Zamparelli A, Vancheri C, Sgalla G, Richeldi L, Cossarizza A
    BMC Med, 2015-11-09;13(0):277.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Heterochromatin Protein 1 Alpha (HP1alpha: CBX5) is a Key Regulator in Differentiation of Endothelial Progenitor Cells to Endothelial Cells.
    Authors: Maeng Y, Kwon J, Kim E, Kwon Y
    Stem Cells, 2015-05-01;33(5):1512-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
    Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
    J. Immunol., 2009-12-15;183(12):8176-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells.
    Authors: Xiong YQ, Sun HC, Zhang W
    Clin. Cancer Res., 2009-07-28;15(15):4838-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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