Human vWF-A2 Antibody

Catalog #: AF2764
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2764
AF2764-SP

Detection of Human vWF‑A2 by Western Blot.

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All vWF-A2 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human vWF-A2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human vWF-A2 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human vWF-A2 Asp1498-Val1665 Accession # NP_000543

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

HUVEC human umbilical vein endothelial cells

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human vWF-A2 (Catalog # 2764‑WF), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human vWF‑A2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human vWF-A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2764) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for vWF at approximately 250 kDa and the vWF precursor at 360-460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human vWF‑A2 by Simple Western^TM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for vWF‑A2 at approximately 250-350 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human vWF‑A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2764). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: vWF-A2

von Willebrand Factor (vWF) is a large, multimeric glycoprotein made by endothelial cells and megakaryocytes. The pre-pro-vWF protein contains 2813 amino acids (aa), which consists of 22 aa signal peptide, 741 aa propeptide and mature vWF monomer of 2050 aa (1-4). The pro-vWF undergoes dimerization in the endoplasmic reticulum (ER) through C-terminal “cysteine-knot” (CK) domain. The pro-vWF dimmers are transported to Golgi and form multimers by forming disulfide bond in amino-terminal region of the mature form. The proteolytic processing of pro-region also occurs in Golgi. The matured vWF is stored in Weibel-Pallade bodies in endothelial cells and granules in megakaryocytes and platelets. The unusually-large vWF (ulvWF) multimers released from cells are very efficient in binding to platelets to form thrombus. The population of these highly active ulvWF multimers is controlled by a specific protease, ADAMTS13, which cleaves between residues Tyr1605 and Met1606 in the A2 domain of vWF. In the plasma, vWF appears as a series of large and intermediate multimers with molecular masses from several thousand to 500 kDa. vWF also performs hemostatic functions (3-5). In a high shear-stressed environment, vWF undergoes conformational change to expose a binding site for glycoprotein Ib alpha. As a result, vWF facilitates aggregation of platelets. In addition to platelet binding, vWF binds coagulation factor VIII to increase the lifetime of FVIII in plasma. The purified rhvWF-A2 contains the A2 domain of vWF.

References

Sadler, J. E. (1998) Annu. Rev. Biochem. 67:395.
Ruggeri, Z. M. (2003) Cur. Opin. Hemat. 10:142.
Michiels, J. J. et al. (2006) Clin. Appl. Thromb. Hemost. 12:397.
Groot, E. et al. (2007) Cur. Opin. Hemat. 14:284.
Lenting, P. J. et al. (2007) J. Thromb. Haemos. 5:1353.

Long Name

von Willebrand Factor A2 Domain

Entrez Gene IDs

7450 (Human); 22371 (Mouse); 116669 (Rat)

Alternate Names

F8VWF; VWD; VWF von Willebrand factor; vWFA2; vWF-A2

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Citation for Human vWF-A2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Coronary angiogenic effect of long-term administration of Nigella sativa
Authors: LI Al Asoom
BMC Complement Altern Med, 2017-06-13;17(1):308.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC