Human WT1 Antibody

Catalog #: AF5729
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5729
AF5729-SP

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All WT1 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human WT1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human WT1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human WT1
Met127-Gly249
Accession # P19544

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human WT1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human WT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5729) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for WT1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Intracellular Staining by Flow Cytometry

Detection of WT1 antibody in HL‑60 Human Cell Line antibody by Flow Cytometry.

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Detection of WT1 in HL‑60 Human Cell Line by Flow Cytometry. HL-60 human acute promyelocytic leukemia cell line was stained with Goat Anti-Human WT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5729, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WT1

WT1 (Wilms’ tumor protein 1; also WT33) is a 52‑54 kDa, nuclear member of the EGR C2H2‑type zinc‑finger family of proteins. Although its predicted MW is 49 kDa, it runs anomalously in SDS‑PAGE, likely due to a high proline content. It is widely expressed, being found in developing Sertoli cells, glomerular podocytes, neurons, and CD34+ stem cells. Human WT1 is 449 amino acids (aa) in length. It contains a Pro‑rich domain (aa 27‑83) and four consecutive C2H2 zinc finger regions
(aa 323‑347; 353‑377; 383‑405; 414‑438). WT1 forms homodimers, and interacts with multiple molecules. Interaction with the zinc fingers generally promotes gene transcription, while N‑terminal interactions block gene transcription. There are at least two dozen splice variants. Some are combinations of deletions of aa 250‑266 and 408‑410, plus an alternate start site 68 aa upstream of the standard site, and a three aa substitution for aa 1‑147. Over aa 127‑249, human WT1 shares 98% aa identity with mouse WT1.

Long Name

Wilms Tumor 1

Entrez Gene IDs

7490 (Human); 22431 (Mouse); 24883 (Rat)

Alternate Names

AWT1; GUD; NPHS4GUD; WAGR; Wilms tumor 1; Wilms tumor protein; WIT-2; WT1; WT33

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human WT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Differentiation of human iPSCs into functional podocytes
Authors: C Rauch, E Feifel, G Kern, C Murphy, F Meier, W Parson, M Beilmann, P Jennings, G Gstrauntha, A Wilmes
PLoS ONE, 2018-09-17;13(9):e0203869.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Renal progenitors derived from human iPSCs engraft and restore function in a mouse model of acute kidney injury.
Authors: Imberti, Barbara, Tomasoni, Susanna, Ciampi, Osele, Pezzotta, Anna, Derosas, Manuela, Xinaris, Christod, Rizzo, Paola, Papadimou, Evangeli, Novelli, Rubina, Benigni, Ariela, Remuzzi, Giuseppe, Morigi, Marina
Sci Rep, 2015-03-06;5(0):8826.
Species: Human
Sample Types: Whole Cells
Applications: IHC
A novel source of cultured podocytes.
Authors: Da Sacco, Stefano, Lemley, Kevin V, Sedrakyan, Sargis, Zanusso, Ilenia, Petrosyan, Astgik, Peti-Peterdi, Janos, Burford, James, De Filippo, Roger E, Perin, Laura
PLoS ONE, 2013-12-12;8(12):e81812.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry