Human WTX Antibody

Catalog #: MAB7374 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7374
MAB7374-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human WTX Antibody Summary

Species Reactivity

Human

Specificity

Detects human WTX in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 729419

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human WTX
Met421-Leu573
Accession # Q5JTC6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human WTX by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for WTX at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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WTX in BG01V Human Embryonic Stem Cells. WTX was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WTX

WTX (Wilms Tumor on the X; also FAM123B and AMER1) is a 190-200 kDa member of the FAM123 family of proteins. It is widely expressed in both adult and embryo and appears to have at least three functions: one, it binds WT1 in the nucleus and regulates cell differentiation; two, it associates with the plasma membrane and draws APC away from microtubules, thereby participating in intercellular junction integrity; and three, it binds beta -catenin and impacts WNT signaling. Human WTX is 1135 amino acids in length. It contains a PtdIns-P2 binding site (aa 2-209), an acidic domain (aa 370-411), and a Pro-rich region (aa 952-1104). There are three APC binding motifs (aa 280-368; 380-431; 717-834) and the beta -catenin binding region lies C-terminal to Gly368. There are two isoform variants. One is 150 kDa in size, nuclear in location, and shows a deletion of aa 50-326. A second shows a 19 aa substitution for aa 786-1135. Over aa 421-573, human WTX shares 89% aa identity with mouse WTX.

Long Name

Wilms Tumor on the X Chromosome

Entrez Gene IDs

139285 (Human); 72345 (Mouse); 501584 (Rat)

Alternate Names

AMER1; FAM123B; OSCS; WTX