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Janelia Fluor® 549, Maleimide

Catalog #: 6500 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
6500/2
Janelia Fluor® 549, Maleimide | Sulfhydyl-Reactive Janelia Fluor Dyes
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Description: Yellow dye (Reactive group: maleimide). Applications: super resolution microscopy e.g. dSTORM & STED, confocal, flow and live cell imaging

Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate

Purity: ≥95%

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Biological Activity

Janelia Fluor® 549, Maleimide is a yellow fluorescent dye, supplied with a maleimide reactive group for conjugation (thiol reactivity). Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1; A280 correction factor is 0.169.

Please see the product protocol for further information and a guide to protein/antibody labeling.

Optical Data for Janelia Fluor® 549, Maleimide

Janelia Fluor<sup>®</sup> 549, Maleimide Dye Spectra
Emission Color Yellow
Brightness
λabs 549 nm
λem 571 nm
Extinction Coefficient (ε) 101,000 M-1cm-1
Quantum Yield (φ) 0.88
Closest Laser line 532 nm
Reactive Group Maleimide
Reactivity Thiols
Cell Permeable Yes
Correction Factor 280 0.169
Application Confocal imaging, dSTORM, STED, Flow
Alternative For Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data

M.Wt:
690.63
Formula:
C33H28N4O6.C2HF3O2
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥95%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Background References

  1. A general method to improve fluorophores for live-cell and single-molecule microscopy
    Grimm et al.
    Nat.Methods., 2015;12:244

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