Home / Fluorescent Dyes / JFX™ 650, Maleimide

JFX™ 650, Maleimide

Catalog #: 8162 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8162/200U
JFX™ 650, Maleimide | Fluorescent Dyes
1 Image
Description: Deuterated Janelia Fluor® dye (Reactive group: Maleimide). Application: confocal microscopy, super resolution microscopy (SRM) including dSTORM & STED. Suitable for live cell imaging

Chemical Name: 2-(5,5-Dimethyl-3-(pyrrolidin-1-ium-1-ylidene-d8)-7-(pyrrolidin-1-yl-d8)-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)carbamoyl)benzoate trifluoroacetate

Purity: ≥90%

Product Details
Reviews
Biological Activity Technical Data Additional Information Product Datasheets Calculators

Biological Activity

Key Information: JFX™ 650, Maleimide is a red fluorescent deuterated Janelia Fluor® dye; supplied with a Maleimide reactive group for conjugation (thiol reactivity). Suitable for live cell imaging.

Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.

Properties and Photophysical Data: JFX™ 650, Maleimide shows enhanced brightness, photostability and chromostability. Maleimide can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 650 nm and 667 nm, respectively; quantum yield = 0.53; extinction coefficient = 17,600 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.015.

Please see the product protocol for further information and a guide to protein/antibody labeling.

We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.

JFX is a trademark owned by the Howard Hughes Medical Institute.

Optical Data for JFX™ 650, Maleimide

λabs 650 nm
λem 667 nm
Extinction Coefficient (ε) 17600 M-1cm-1
Quantum Yield (φ) 0.53

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

Technical Data

M.Wt:
776.94
Formula:
C37H22D16N4O5Si.CF3CO2H
Purity:
≥90%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Molarity Calculator

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

FAQs

No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for JFX™ 650, Maleimide

There are currently no reviews for this product. Be the first to review JFX™ 650, Maleimide and earn rewards!

Have you used JFX™ 650, Maleimide?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.