JFX™ 650, NHS ester New
Chemical Name: 2-(5,5-Dimethyl-3-(pyrrolidin-1-ium-1-ylidene-d8)-7-(pyrrolidin-1-yl-d8)-3,5-dihydrodibenzo[b,e]silin-10-yl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)benzoate
Purity: ≥90%
Biological Activity
Key Information: JFX™ 650, NHS ester is a red fluorescent deuterated Janelia Fluor® dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.Application: Suitable for confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Cell permeable.
Properties and Photophysical Data: JFX™ 650, NHS ester shows enhanced brightness, photostability and chromostability. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 650 nm and 667 nm, respectively; quantum yield = 0.53; extinction coefficient = 17,600 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.015.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
JFX is a trademark owned by the Howard Hughes Medical Institute.
Optical Data for JFX™ 650, NHS ester
| λabs | 650 nm |
|---|---|
| λem | 667 nm |
| Extinction Coefficient (ε) | 17,600 M-1cm-1 |
| Quantum Yield (φ) | 0.53 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, dSTORM, STED, Flow Cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
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