L-Azidohomoalanine hydrochloride

Biological Activity

L-Azidohomoalanine hydrochloride is an unnatural amino acid. Incorporated into proteins during de novo protein synthesis, with no effect on protein degradation. Requires methionine-free medium for efficient incorporation. Substrate for wild-type methionyl-tRNA synthetase (MetRS) in vivo. Can be used in BONCAT (bio-orthogonal non-canonical amino acid tagging) for the identification of newly synthesized proteins.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT).
   Dieterich et al.
   Proc.Natl.Acad.Sci.U.S.A., 2006;103:9482
2. Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins.
   Lang & Chin et al.
   Chem.Rev., 2014;114:4764
3. Nonradioactive quantification of autophagic protein degradation with L-azidohomoalanine labeling.
   Wang et al.
   Nat.Protoc., 2017;12:279

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