When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

Name: MagCellect Human CD14+ Cell Isolation Kit
Brand: R&D Systems
SKU: MAGH105
Price: 659 USD

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Human CD14+ Cell Isolation Kit

Catalog #: MAGH105
6 Citations Datasheet / COA / SDS

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MAGH105

R&D Systems Magcellect Cell Isolation Kits

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Citations (6)

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MagCellect Human CD14+ Cell Isolation Kit Summary

Specifications

Shipping Conditions

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Species

Human

Product Datasheets

Product Datasheet

COA

SDS

Background: CD14

CD14 is an acute phase glycoprotein that binds lipopolysaccharide endotoxins (LPS) from Gram-negative bacteria. It is primarily expressed on monocytes, macrophages, and neutrophils, and soluble forms can be secreted or shed from the cell surface. CD14 accepts LPS from its initial binding by LBP, and it can further transfer LPS to the TLR4/MD2 complex. LPS acctivation of TLR4 triggers inflammatory activation. CD14 also contributes to the response to TLR3 ligands, phosphoinositides, as well as the surfactant proteins SP-A and SP-D.

Entrez Gene IDs

929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

Alternate Names

CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

Related Research Areas

Citations for MagCellect Human CD14+ Cell Isolation Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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The Pseudogene Olfr29-ps1 Promotes the Suppressive Function and Differentiation of Monocytic MDSCs
Authors: W Shang, Y Gao, Z Tang, Y Zhang, R Yang
Cancer Immunol Res, 2019-03-26;0(0):. 2019-03-26
Melanoma Cells Can Adopt the Phenotype of Stromal Fibroblasts and Macrophages by Spontaneous Cell Fusion in Vitro
Authors: Lajos V Kemény
Int J Mol Sci, 2016-06-02;17(6):. 2016-06-02
Pharmacological Inhibition of Protein Kinase G1 Enhances Bone Formation by Human Skeletal Stem Cells Through Activation of RhoA-Akt Signaling.
Authors: Jafari A, Siersbaek M, Chen L, Qanie D, Zaher W, Abdallah B, Kassem M
Stem Cells, 2015-04-23;33(7):2219-31. 2015-04-23
Glucocorticoids maintain human osteoclasts in the active mode of their resorption cycle.
Authors: Soe K, Delaisse JM
J. Bone Miner. Res., 2010-10-01;25(10):2184-92. 2010-10-01
Reduced ex vivo interleukin-6 production by dietary fish oil is not modified by linoleic acid intake in healthy men.
Authors: Damsgaard CT, Lauritzen L, Calder PC, Kjaer TR, Frokiaer H
J. Nutr., 2009-06-03;139(7):1410-4. 2009-06-03
Pulse treatment with the proteasome inhibitor bortezomib inhibits osteoclast resorptive activity in clinically relevant conditions.
Authors: Boissy P, Andersen TL, Lund T, Kupisiewicz K, Plesner T, Delaisse JM
Leuk. Res., 2008-04-18;32(11):1661-8. 2008-04-18

FAQs

When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?
- It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

View all Cell Selection and Detection Kit FAQs