Mouse ACE-2 Antibody

Detection of Mouse ACE‑2 by Western Blot. Western blot shows lysates of mouse kidney, mouse ovary, mouse heart, and mouse testis. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34372) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for ACE‑2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of ACE-2 in HEK293 Human Cell Line Transfected with Mouse ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse ACE-2 or (B) irrelevant protein, and eGFP was stained with Rabbit Anti-Mouse ACE-2 Monoclonal Antibody (Catalog # MAB34372) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol.

ACE‑2 in HEK293 Human Cell Line transfected with ACE-2. ACE‑2 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with ACE-2 (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Rabbit Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34372) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse ACE-2 by Simple Western Expression of hACE2 in hACE2-KI mouse model. (A) The expression pattern of hACE2 mRNA in different organs including brain, heart, intestines, kidneys, liver, lungs, spleen, stomach, and trachea was detected by real-time quantitative PCR (qPCR) assay. (B) Representative immunoblotting image shows the expression of the ACE2 protein in mouse intestine and lung tissues normalized to total protein. Human ACE2 was highly expressed in the lungs and intestines of hACE2-KI mice, while mouse ACE2 protein was detected in WT mice but not in hACE2-KI mice. (C) Immunochemistry results of the hACE2 expression in different organs. The intestines, lungs, and trachea sections are shown in high-power magnification; scale bar: 100 μm. Statistical analysis was performed using unpaired Student’s t-test or Wilcoxon rank test. NS: not significant; *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37350787), licensed under a CC-BY license. Not internally tested by R&D Systems.