Mouse Angiopoietin-like Protein 4/ANGPTL4 Antibody

Catalog #: MAB97971 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB97971-100
MAB97971-SP

All Angiopoietin-like Protein 4/ANGPTL4 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Angiopoietin-like Protein 4/ANGPTL4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Angiopoietin-like Protein 4/ANGPTL4 in direct Elisa.

Source

Monoclonal Rat IgG_2A Clone # 547113

Purification

Protein A or G purified from cell culture supernatant

Immunogen

CHO-derived mouse Angiopoietin-like Protein 4/ANGPTL4 protein

Gln24-Ser410
Accession # Q9Z1P8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an Angiopoietin‑like Protein 4/ANGPTL4 ELISA capture antibody when paired with Catalog # MAB9797

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Mature mouse ANGPTL4 shares 26%-30% amino acid (aa) sequence identity with mouse ANGPTL1, 2, 3, 4, 6, and 7. It shares 75% and 97% aa sequence identity with human and rat ANGPTL4, respectively. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).

References

Zhu, P. et al. (2012) Biosci. Rep. 32:211.
Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
Cazes, A. et al. (2006) Circ. Res. 99:1207.
Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
Hou, M. et al. (2014) PLoS ONE 9:e85808.
Clement, L.C. et al. (2011) Nat. Med. 17:117.

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Alternate Names

Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein