Mouse B7-1/CD80 Antibody Summary
Val38-Asn246
Accession # Q00609
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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B7-1/CD80 in Mouse Colon Tissue. B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of mouse colon tissue using Rat Anti-Mouse B7-1/CD80 Monoclonal Antibody (Catalog # MAB740) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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B7‑1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rat Anti-Mouse B7-1/CD80 Monoclonal Antibody (Catalog # MAB740) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-1/CD80
B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 and B7-2 share 28% amino acid identity. Mouse and human B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.
- Azuma, M. et al. (1993) Nature 366:76.
- Freeman, G.J. et al. (1993) Science 262:909.
- Freeman, G. et al. (1991) J. Exp. Med. 174:625.
- Selvakumar, A. et al. (1993) Immunogenetics 38:292.
- Chen, C. et al. (1994) J. Immunol. 152:4929.
- Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Product Datasheets
Citations for Mouse B7-1/CD80 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Macrophage polarity and wound age determination
Authors: Y Kuninaka, Y Ishida, A Ishigami, M Nosaka, J Matsuki, H Yasuda, A Kofuna, A Kimura, F Furukawa, T Kondo
Scientific Reports, 2022-11-25;12(1):20327.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cystatin C Plays a Sex-Dependent Detrimental Role in Experimental Autoimmune Encephalomyelitis
Authors: V Hoghooghi, AL Palmer, A Frederick, Y Jiang, JE Merkens, A Balakrishn, TM Finlay, A Grubb, E Levy, P Gordon, FR Jirik, MD Nguyen, C Schuurmans, F Visser, SE Dunn, SS Ousman
Cell Rep, 2020-10-06;33(1):108236.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Mechanistic dissection of the PD-L1:B7-1 co-inhibitory immune complex
Authors: SC Garrett-Th, A Massimi, EV Fedorov, JB Bonanno, L Scandiuzzi, B Hillerich, RD Seidel, JD Love, SJ Garforth, C Guha, SC Almo
PLoS ONE, 2020-06-04;15(6):e0233578.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice
Authors: J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji
EBioMedicine, 2018-09-15;35(0):29-39.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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