Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
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Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Mouse C-Reactive Protein/CRP Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.015 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse CRP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse C-Reactive Protein/CRP immunoassay is a 4.5 hour solid-phase ELISA designed to measure CRP in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse CRP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse CRP. Results obtained using natural mouse CRP showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse CRP.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.453 | 1.31 | 3.78 | 0.48 | 1.33 | 3.76 |
| Standard Deviation | 0.035 | 0.083 | 0.089 | 0.052 | 0.087 | 0.314 |
| CV% | 7.7 | 6.3 | 2.4 | 10.8 | 6.5 | 8.4 |
Recovery
The recovery of mouse CRP spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 82-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse CRP in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: C-Reactive Protein/CRP
C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.
Entrez Gene IDs:
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names:
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Citations for Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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?-catenin mRNA encapsulated in SM-102 lipid nanoparticles enhances bone formation in a murine tibia fracture repair model
Authors: Nelson, AL;Mancino, C;Gao, X;Choe, JA;Chubb, L;Williams, K;Czachor, M;Marcucio, R;Taraballi, F;Cooke, JP;Huard, J;Bahney, C;Ehrhart, N;
Bioactive materials
Species: Mouse
Sample Types: Serum
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microRNA blood signature for localized radiation injury
Authors: Ancel, L;Gabillot, O;Szurewsky, C;Granger, R;Sache, A;Voyer, F;Gruel, G;Illiano, S;Benderitter, M;Le Guen, B;Souidi, M;Benadjaoud, MA;Flamant, S;
Scientific reports
Species: Mouse
Sample Types: Plasma
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Genistein attenuated oxidative stress, inflammation, and apoptosis in L-arginine induced acute pancreatitis in mice
Authors: P Siriviriya, J Sriko, K Somanawat, M Chayanupat, N Klaikeaw, D Werawatgan
BMC complementary medicine and therapies, 2022-08-04;22(1):208.
Species: Mouse
Sample Types: Serum
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Actions of Esomeprazole on the Maternal Vasculature in Lean and Obese Pregnant Mice with Impaired Nitric Oxide Synthesis: A Model of Preeclampsia
Authors: N de Alwis, NK Binder, YTM Mangwiro, S Beard, N Pritchard, E Kadife, BR Fato, E Keenan, FC Brownfoot, TJ Kaitu'u-Li, NJ Hannan
International Journal of Molecular Sciences, 2022-07-25;23(15):.
Species: Mouse
Sample Types: Serum
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Pirfenidone increases IL10 and improves acute pancreatitis in multiple clinically relevant murine models
Authors: E Palathinga, J George, M Tarique, S Iyer, P Sahay, B Gomez Agui, DB Edwards, B Giri, V Sethi, T Jain, P Sharma, U Vaish, HK C Jacob, A Ferrantell, CL Maynard, AK Saluja, RK Dawra, V Dudeja
JCI Insight, 2022-01-25;0(0):.
Species: Mouse
Sample Types: Serum
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Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?
Authors: Z Zhang, H Na, Q Gan, Q Tao, Y Alekseyev, J Hu, Z Yan, JB Yang, H Tian, S Zhu, Q Li, IM Rajab, JK Blusztajn, B Wolozin, A Emili, X Zhang, T Stein, LA Potempa, WQ Qiu
Aging Cell, 2021-10-23;0(0):e13501.
Species: Mouse
Sample Types: Serum
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Pleiotropic Effects of Atorvastatin Result in a Downregulation of the Carboxypeptidase U System (CPU, TAFIa, CPB2) in a Mouse Model of Advanced Atherosclerosis
Authors: K Claesen, L Roth, JC Mertens, K Hermans, Y Sim, D Hendriks
Pharmaceutics, 2021-10-19;13(10):.
Species: Mouse
Sample Types: Plasma
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Circulating Endothelial Progenitor Cells Are Preserved in Female Mice Exposed to Ambient Fine Particulate Matter Independent of Estrogen
Authors: X Liu, Y Xiao, Q Zhu, Y Cui, H Hao, M Wang, PJ Cowan, RJ Korthuis, G Li, Q Sun, Z Liu
International Journal of Molecular Sciences, 2021-07-04;22(13):.
Species: Mouse
Sample Types: Serum
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An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage
Authors: JK Nam, AR Kim, SH Choi, JH Kim, KJ Choi, S Cho, JW Lee, HJ Cho, YW Kwon, J Cho, KS Kim, J Kim, HJ Lee, TS Lee, S Bae, HJ Hong, YJ Lee
Nature Communications, 2021-06-02;12(1):3279.
Species: Mouse
Sample Types: Serum
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Inflammation-driven senescence-associated secretory phenotype in cancer-associated fibroblasts enhances peritoneal dissemination
Authors: T Yasuda, M Koiwa, A Yonemura, K Miyake, R Kariya, S Kubota, T Yokomizo-N, N Yasuda-Yos, T Uchihara, R Itoyama, L Bu, L Fu, K Arima, D Izumi, S Iwagami, K Eto, M Iwatsuki, Y Baba, N Yoshida, H Ohguchi, S Okada, K Matsusaki, G Sashida, A Takahashi, P Tan, H Baba, T Ishimoto
Cell Reports, 2021-02-23;34(8):108779.
Species: Mouse
Sample Types: Serum
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Metformin treatment of high-fat diet-fed obese male mice restores sperm function and fetal growth, without requiring weight loss
Authors: NO McPherson, M Lane
Asian J. Androl., 2020-11-01;0(0):.
Species: Mouse
Sample Types: Plasma
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Lactobacillus fermentum promotes adipose tissue oxidative phosphorylation to protect against diet-induced obesity
Authors: Y Yoon, G Kim, MG Noh, JH Park, M Jang, S Fang, H Park
Exp. Mol. Med., 2020-09-11;0(0):.
Species: Mouse
Sample Types: Serum
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Anti-Inflammatory and Gut Microbiota Modulatory Effect of Lactobacillus rhamnosus Strain LDTM 7511 in a Dextran Sulfate Sodium-Induced Colitis Murine Model
Authors: S Yeo, H Park, E Seo, J Kim, BK Kim, IS Choi, CS Huh
Microorganisms, 2020-06-04;8(6):.
Species: Mouse
Sample Types: Serum
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Development of insulin resistance preceded major changes in iron homeostasis in mice fed a high-fat diet
Authors: J Varghese, JV James, R Anand, M Narayanasa, G Rebekah, B Ramakrishn, AJ Nellickal, M Jacob
J. Nutr. Biochem., 2020-06-02;84(0):108441.
Species: Mouse
Sample Types: Serum
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HIF1alpha inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis
Authors: C Liang, J Li, C Lu, D Xie, J Liu, C Zhong, X Wu, R Dai, H Zhang, D Guan, B Guo, B He, F Li, X He, W Zhang, BT Zhang, G Zhang, A Lu
Nat Commun, 2019-10-08;10(1):4579.
Species: Mouse
Sample Types: Serum
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REDD-1 aggravates endotoxin-induced inflammation via atypical NF-?B activation
Authors: DK Lee, JH Kim, J Kim, S Choi, M Park, W Park, S Kim, KS Lee, T Kim, J Jung, YK Choi, KS Ha, MH Won, TR Billiar, YG Kwon, YM Kim
FASEB J., 2018-03-16;0(0):fj201701436R.
Species: Mouse
Sample Types: Serum
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Berberine inhibits the ischemia-reperfusion injury induced inflammatory response and apoptosis of myocardial cells through the phosphoinositide 3-kinase/RAC-? serine/threonine-protein kinase and nuclear factor-?B signaling pathways
Authors: L Wang, H Ma, Y Xue, H Shi, T Ma, X Cui
Exp Ther Med, 2017-11-27;15(2):1225-1232.
Species: Mouse
Sample Types: Serum
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Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
By Anonymous
on 02/25/2023
Sample Tested: Serum and Plasma
