Mouse Carbohydrate Sulfotransferase 7/CHST7 Antibody

Catalog #: AF5108 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5108
AF5108-SP

All Carbohydrate Sulfotransferase 7/CHST7 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Carbohydrate Sulfotransferase 7/CHST7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Sulfotransferase 7/CHST7 in direct ELISAs and Western blots. In direct ELISA, less than 1% cross-reactivity with recombinant mouse (rm) CHST1, recombinant human CHST2, rmCHST5, and rmCHST3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CHST7
Ser34-Val484
Accession # Q9EP78

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 (Catalog # 5108-ST)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 (Catalog # 5108-ST), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 7/CHST7

The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST7, also designated as chondroitin 6-O-sulfotransferase (C6ST-2), N-acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST-4) and Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST-5), was previously shown to act on chondroitin sulfate, mannose-linked GlcNAc and mucin associated GlcNAc residues (3‑5). The amino acid sequence of mouse CHST7 is 87% identical to that of the human counterpart.

References

Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
Kitagawa, H. et al. (2000) J. Biol. Chem. 275:21075.
Uchimura, K. et al. (2000) Biochem. Biophys. Res. Commun. 274:291.
Bhakta, S. et al. (2000) J. Biol. Chem. 275:40226.
Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, p.964.
MacRae, I.J. et al. (2000) Biochemistry 39:1613.
Wu, Z.L. et al. (2002) FASEB J. 16:539.