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Mouse CCL20/MIP-3 alpha Antibody

Catalog #: AF760
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF760
AF760-SP
Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL20/MIP-3 alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL20/MIP-3 alpha in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL20/MIP-3 alpha
Ala27-Met96
Accession # Q642U4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL20/MIP‑3 alpha (Catalog # 760-M3)
Neutralization
Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL20/MIP‑3 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody. View Larger

Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody. Recombinant Mouse CCL20/MIP-3a (760-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP-3a (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL20/MIP-3a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF760). The ND50 is typically 0.5-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

MIP-3 alpha, also known as LARC (Liver and Activation-regulated Chemokine) and Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20-28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71% and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively.

MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

Entrez Gene IDs
6364 (Human); 20297 (Mouse); 29538 (Rat)
Alternate Names
beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Citations for Mouse CCL20/MIP-3 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Chronic Inflammation Directs an Olfactory Stem Cell Functional Switch from Neuroregeneration to Immune Defense
    Authors: M Chen, RR Reed, AP Lane
    Cell Stem Cell, 2019-09-12;25(4):501-513.e5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-?B signaling
    Authors: T Kanaya, S Sakakibara, T Jinnohara, M Hachisuka, N Tachibana, S Hidano, T Kobayashi, S Kimura, T Iwanaga, T Nakagawa, T Katsuno, N Kato, T Akiyama, T Sato, IR Williams, H Ohno
    J. Exp. Med., 2018-01-16;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. The Effect of Cigarette Smoke Exposure on the Development of Inflammation in Lungs, Gut and Joints of TNFDeltaARE Mice.
    Authors: Allais L, Kumar S, Debusschere K, Verschuere S, Maes T, De Smet R, Conickx G, De Vos M, Laukens D, Joos G, Brusselle G, Elewaut D, Cuvelier C, Bracke K
    PLoS ONE, 2015-11-02;10(11):e0141570.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
    Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
    J Invest Dermatol, 2012-08-16;133(1):164-71.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. CCL20, gammadelta T cells, and IL-22 in corneal epithelial healing.
    Authors: Li Z, Burns AR, Miller SB, Smith CW
    FASEB J., 2011-04-25;25(8):2659-68.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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