Mouse CCR5 Antibody

Catalog #: MAB6138
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6138
MAB6138-SP

Detection of Mouse CCR5 by Western Blot.

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All CCR5 Antibodies

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Citations (1)

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Mouse CCR5 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects CCR5 in direct ELISAs and Western blots. No cross-reactivity with recombinant mouse CCR1, 4, or 8 is observed.

Source

Monoclonal Rat IgG₁ Clone # 645807

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant mouse CCR5
Met1-Ala32
Accession # P51682

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

RAW 264.7 mouse monocyte/macrophage cell line treated with LPS under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CCR5 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line treated (+) with 100 µg/mL LPS for 4 hours. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse CCR5 Monoclonal Antibody (Catalog # MAB6138) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCR5 at approximately 50 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 (CC chemokine receptor 5; also CD195 and MIP-1a receptor) is a 41 - 44 kDa member of the G-protein coupled receptor #1 family of proteins. It is expressed on Treg cells, NK cells, neurons, macrophages and Th1 cells. CCR5 mediates cell adhesion and migration induced by several chemokines including CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL5/RANTES, and CCL8/MCP-2. It also functions as a coreceptor for macrophage-tropic HIV-1 infection. CCR5 contains an O-glycosylated and sulfated extracellular N-terminus (aa 1 - 32), and a phosphorylated and palmitoylated intracellular C-terminus (aa 304 - 354). CCR5 will form homodimers, heterodimers with CCR2, and heterooligomers with CCR2 and CXCR4. Within aa 1 - 32, mouse CCR5 shares 72% and 91% amino acid sequence identity with human and rat CCR5, respectively.

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Alternate Names

C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

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Citation for Mouse CCR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1