Mouse CD117/c-kit APC-conjugated Antibody

Catalog #: FAB1356A
1 Citation Datasheet / COA / SDS

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FAB1356A

Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry.

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All CD117/c-kit Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD117/c-kit APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD117/c-kit in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 180627

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit APC-conjugated Monoclonal Antibody (Catalog # FAB1356A) and Rat Anti-Mouse Sca-1/Ly6 PE-conjugated Monoclonal Antibody (Catalog # FAB1226P). Quadrant markers were set based on control antibody staining (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD117/c-kit

SCF R is also known as the c-kit proto-oncogene protein and as CD117. Together with its ligand, stem cell factor (also called c-kit ligand or mast cell growth factor), SCF R plays an essential role in gametogenesis, melanogenesis, and hematopoiesis. SCF R is expressed in hematopoietic progenitor cells, normal B and T lymphocyte progenitors, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF R expression has also been reported in several human tumor cell lines (1,2).

References

Broudy, V. (1997) Blood 90:1345.
Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.

Long Name

Stem Cell Factor Receptor

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Alternate Names

CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse CD117/c-kit APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Maternal high-fat diet results in microbiota-dependent expansion of ILC3s in mice offspring
Authors: ST Babu, X Niu, M Raetz, RC Savani, LV Hooper, J Mirpuri
JCI Insight, 2018-10-04;3(19):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry