Mouse CD117/c-kit Biotinylated Antibody

Catalog #: BAF1356
2 Citations Datasheet / COA / SDS

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BAF1356

All CD117/c-kit Antibodies

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Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD117/c-kit Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD117/c-kit in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human CD117 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD117/c-kit

Flow Cytometry

0.25 µg/10⁶ cells

Lineage depleted mouse bone marrow

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (E15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.

Long Name

Stem Cell Factor Receptor

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Alternate Names

CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CD117/c-kit Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution
Authors: T Chakrabort, MK Driscoll, E Jeffery, MM Murphy, P Roudot, BJ Chang, S Vora, WM Wong, CD Nielson, H Zhang, V Zhemkov, C Hiremath, ED De La Cruz, Y Yi, I Bezprozvan, H Zhao, R Tomer, R Heintzmann, JP Meeks, DK Marciano, SJ Morrison, G Danuser, KM Dean, R Fiolka
Nat. Methods, 2019-10-31;16(11):1109-1113.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC