Mouse CD11c Antibody

Catalog #: MAB69501
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB69501
MAB69501-SP

Detection of CD11c in Mouse Splenocytes by Flow Cytometry.

1 Image

All CD11c Antibodies

Product Details

Citations (4)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD11c Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD11c.

Source

Monoclonal Hamster IgG Clone # N418

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse spleen dendritic cells

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-reported

Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD11c in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Hamster Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB69501) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody (Catalog # F0120) and Rat Anti-Mouse MHC class II (I-A/I-E) APC-conjugated Monoclonal Antibody (Catalog # FAB6118A). Quadrant markers were set based on normal hamster IgG control antibody staining.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11c

CD11c, also known as the Integrin alpha X subunit, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the beta 2 subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages, dendritic cells, hairy cell leukemias and some other leukocyte subsets. The 1097 aa mouse CD11c extracellular domain shares 71% and 87% amino acid (aa) identity with human and rat CD11c, respectively. One potential alpha X isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are shared with alpha M beta 2/CD11b/CD18 (Complement iC3b, ICAMs, vWF and Fibrinogen) while others (Osteopontin, Thy-1, Plasminogen, Heparin) are unique. Unlike alpha M beta 2, it is not constitutively active. alpha X beta 2 adhesion mediates proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles.

References

Metlay, J.P. et al. (1990) J. Exp. Med. 171:1753.

Entrez Gene IDs

3687 (Human); 16411 (Mouse); 64350 (Rat)

Alternate Names

95 alpha chain; 95; 95, alpha subunit; CD11 antigen-like family member C; CD11c antigen; CD11c; CD11Cleu M5, alpha subunit; Integrin alpha X; integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAX; Leu M5; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150; leukocyte surface antigen p150; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; p150,95 alpha; SLEB6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CD11c Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
Sci Rep, 2016-08-22;6(0):31874.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
In vivo adjuvant-induced mobilization and maturation of gut dendritic cells after oral administration of cholera toxin.
Authors: Anjuere F, Luci C, Lebens M, Rousseau D, Hervouet C, Milon G, Holmgren J, Ardavin C, Czerkinsky C
J Immunol, 2004-10-15;173(8):5103-11.
Species: Mouse
Sample Types: Whole Cells
Applications: FACS
Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover.
Authors: Leenen P, Radosevic K, Voerman J, Salomon B, Van Rooijen N, Klatzmann D, van Ewijk W
J Immunol, 1998-03-01;160(5):2166-73.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Functional comparison of spleen dendritic cells and dendritic cells cultured in vitro from bone marrow precursors.
Authors: Garrigan K, Moroni-Rawson P, McMurray C, Hermans I, Abernethy N, Watson J, Ronchese F
Blood, 1996-11-01;88(9):3508-12.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry