Mouse CD27/TNFRSF7 Antibody

Catalog #: MAB5741 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5741
MAB5741-SP

Detection of CD27/TNFRSF7 in Mouse Splenocytes by Flow Cytometry.

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All CD27/TNFRSF7 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD27/TNFRSF7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD27 in direct ELISAs and Western blots. In Western blots; does not cross-react with recombinant human (rh) CD27, recombinant mouse (rm) CD40, rmGITR, rhDR6, rhTRAIL R4, rmHVEM, rmCD30, rmRANK, rmFas, rmTRAIL R2, rhTRADD, rm4-1BB, rmLT beta R, rmOPG, rmNGFR, rmDR3, rmBAFF R, rhTRAIL R3, rmTAJ, rmEDAR, rmOX-40, rmTWEAK R, rmTNF R1, rhRELT, or rmTRAIL R1.

Source

Monoclonal Rat IgG_2A Clone # 137915

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD27/TNFRSF7
Thr21-Arg182
Accession # P41272

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Mouse CD27/TNFRSF7 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse CD27/TNFRSF7 Biotinylated Antibody (Catalog # BAM5741)

Standard: Recombinant Mouse CD27/TNFRSF7 Fc Chimera Protein, CF (Catalog # 574-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD27/TNFRSF7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were labeled with Mouse CD27/TNFRSF7 Monoclonal Antibody (Catalog # MAB5741) followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113) and Mouse CD3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4841F). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

Immunocytochemistry

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CD27/TNFRSF7 in Mouse Splenocytes. CD27/TNFRSF7 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD27/TNFRSF7 Monoclonal Antibody (Catalog # MAB5741) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD27/TNFRSF7

CD27 is a lymphocyte-specific member of the tumor necrosis factor receptor superfamily (TNFRSF) and is designated TNFRSF7 (1, 2). Mouse CD27 cDNA encodes a 250 amino acid (aa) residue type I transmembrane protein with a 20 aa putative signal peptide, a 162 aa extracellular region containing three TNFR cysteine-rich repeats, a 21 aa transmembrane domain and a 47 aa cytoplasmic region (3). Mouse and human CD27 share approximately 65% amino acid identity. CD27 exists as homodimers on the cell surface via an extracellular disulfide bond in the membrane-proximal region. A soluble form of CD27 is also produced during the immune response and is found in various body fluids (4). CD27 is expressed on subsets of T and B cells. The expression of CD27 is upregulated upon T cell activation. Although CD27 appears to be a marker for human memory B cells, it is only expressed in a small population of mouse B cells in germinal centers and at sites of B cell stimulation, suggesting that mouse CD27 may be a marker for activated B cells (5). CD27 interacts with CD27 ligand (also named CD70 and TNFSF7), which is a member of the TNF ligand superfamily. Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (1, 2). Ligation of CD27 on B cells has been shown to inhibit terminal differentiation of activated mouse B cells into plasma cells and enhances commitment to memory B cell responses (5).

References

Croft, M. (2003) Nature Reviews Immunol. 3:609.
Croft, M. (2003) Cytokine and Growth Factor Reviews 14:265.
Gravestein, L.A. et al. (1993) Eur. J. Immunol. 23:943.
Lens, S.M. et al. (1998) Semin. Immunol. 10:491.
Raman, V.S. et al. (2003) J. Immunol. 171:5876.

Entrez Gene IDs

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Alternate Names

CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7