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Mouse CD3 Antibody

Catalog #: MAB4841
29 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4841-SP
MAB4841-100
MAB4841-500
CD3 in Mouse Splenocytes.
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Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD3 Antibody Summary

Species Reactivity
Mouse
Specificity
Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
Source
Monoclonal Rat IgG2B Clone # 17A2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
T cell hybridoma D1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
Immunohistochemistry
8-25 µg/mL
Perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) and perfusion fixed paraffin-embedded sections of Mouse Thymus
Immunoprecipitation
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
 
Complement-dependent Cytotoxicity
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113 and Wu, L. et al. (1991) J. Exp. Med. 174:1617.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-25 µg/mL
See below
T Cell Stimulation
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD3 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD3 in Mouse Splenocytes. CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD3 antibody in Mouse Brain. View Larger

CD3 in Mouse Brain. CD3 was detected in perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) using 10 µg/mL Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry View Larger

Detection of CD3 in Mouse Thymus. CD3 was detected in perfusion fixed paraffin-embedded sections of Mouse Thymus using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Entrez Gene IDs
916 (Human); 12501 (Mouse)
Alternate Names
CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Citations for Mouse CD3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. ITK signaling regulates a switch between T helper 17 and T regulatory cell lineages via a calcium-mediated pathway
    Authors: O Anannya, W Huang, A August
    bioRxiv : the preprint server for biology, 2023-04-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
    Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
    The Journal of Biological Chemistry, 2022-03-10;0(0):101817.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?
    Authors: Z Zhang, H Na, Q Gan, Q Tao, Y Alekseyev, J Hu, Z Yan, JB Yang, H Tian, S Zhu, Q Li, IM Rajab, JK Blusztajn, B Wolozin, A Emili, X Zhang, T Stein, LA Potempa, WQ Qiu
    Aging Cell, 2021-10-23;0(0):e13501.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  4. The YAP/HIF-1&alpha/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation
    Authors: J Zhou, N Zhang, W Zhang, C Lu, F Xu
    Cell & bioscience, 2021-05-12;11(1):84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Itgb3-integrin-deficient mice may not be a sufficient�model for patients with Glanzmann thrombasthenia
    Authors: D Li, J Peng, T Li, Y Liu, M Chen, X Shi
    Molecular Medicine Reports, 2021-04-21;23(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Hepatocyte cannabinoid 1 receptor nullification alleviates toxin-induced liver damage via NF-&kappaB signaling
    Authors: Y Kim, S Gautam, KR Aseer, J Kim, P Chandrasek, CH Mazucanti, P Ghosh, JF O'Connell, ME Doyle, A Appleton, E Lehrmann, QR Liu, JM Egan
    Cell Death & Disease, 2020-12-09;11(12):1044.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Regulatory T cell phenotype and anti-osteoclastogenic function in experimental periodontitis
    Authors: C Alvarez, S Suliman, R Almarhoumi, ME Vega, C Rojas, G Monasterio, M Galindo, R Vernal, A Kantarci
    Sci Rep, 2020-11-04;10(1):19018.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice
    Authors: J Chida, H Hara, K Uchiyama, E Takahashi, H Miyata, H Kosako, Y Tomioka, T Ito, H Horiuchi, H Matsuda, H Kido, S Sakaguchi
    PLoS Pathog., 2020-08-26;16(8):e1008823.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC, Neutralization
  9. Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy
    Authors: YP Xu, L Lv, Y Liu, MD Smith, WC Li, XM Tan, M Cheng, Z Li, M Bovino, J Aubé, Y Xiong
    J. Clin. Invest., 2019-07-16;130(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Contributions of T cell dysfunction to the resistance against anti-PD-1 therapy in oral carcinogenesis
    Authors: L Wen, H Lu, Q Li, Q Li, S Wen, D Wang, X Wang, J Fang, J Cui, B Cheng, Z Wang
    J. Exp. Clin. Cancer Res., 2019-07-10;38(1):299.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3+ T Cell through Modulation of CD11b+ Cell Function
    Authors: J Wu, J Gu, S Zhou, H Lu, Y Lu, L Lu, X Wang
    J Immunol Res, 2018-08-07;2018(0):1605341.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Activation
  12. CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration
    Authors: MR Deyhle, PS Hafen, J Parmley, CN Preece, M Robison, JR Sorensen, B Jackson, DL Eggett, CR Hancock, RD Hyldahl
    Physiol Rep, 2018-04-01;6(8):e13689.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Nitrosative damage during retrovirus infection-induced neuropathic pain
    Authors: P Chauhan, WS Sheng, S Hu, S Prasad, JR Lokensgard
    J Neuroinflammation, 2018-03-05;15(1):66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Crucial role of OX40/OX40L signaling in a murine model of asthma
    Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
    Mol Med Rep, 2018-01-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  15. A Novel C-Terminal Mutation in Gsdma3 (C+/H-) Leads to Alopecia and Corneal Inflammatory Response in Mice
    Authors: S Swirski, C Röger, A Pienkowska, C Ihlenburg, G Fischer, O May, M Vorm, M Owczarek-L, J Neidhardt
    Invest. Ophthalmol. Vis. Sci., 2018-01-01;59(1):561-571.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression
    Authors: X Fu, F Sun, F Wang, J Zhang, B Zheng, J Zhong, T Yue, X Zheng, JF Xu, CY Wang
    Mediators Inflamm., 2017-09-19;2017(0):5706152.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  17. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
    Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
    Sci Rep, 2017-07-26;7(1):6607.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  18. The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells
    Authors: W Pan, WT Hao, YJ Shen, XY Li, YJ Wang, FF Sun, JH Yin, J Zhang, RX Tang, JP Cao, KY Zheng
    Parasit Vectors, 2017-07-21;10(1):348.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  19. Characterisation of Immune and Neuroinflammatory Changes Associated with Chemotherapy-Induced Peripheral Neuropathy
    Authors: PG Makker, SS Duffy, JG Lees, CJ Perera, RS Tonkin, O Butovsky, SB Park, D Goldstein, G Moalem-Tay
    PLoS ONE, 2017-01-26;12(1):e0170814.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology
    Alzheimers Res Ther, 2016-12-15;8(1):54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCR? Repertoire
    Authors: David A Hildeman
    J. Immunol., 2016-11-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  22. Metronomic Doses of Temozolomide Enhance the Efficacy of Carbon Nanotube CpG Immunotherapy in an Invasive Glioma Model.
    Authors: Ouyang M, White E, Ren H, Guo Q, Zhang I, Gao H, Yanyan S, Chen X, Weng Y, da Fonseca A, Shah S, Manuel E, Zhang L, Vonderfecht S, Alizadeh D, Berlin J, Badie B
    PLoS ONE, 2016-02-01;11(2):e0148139.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.
    Authors: Fan, Miaomiao, Zhang, Yijun, Huang, Zhuoqion, Liu, Jun, Guo, Xuemin, Zhang, Hui, Luo, Haihua
    PLoS ONE, 2014-09-24;9(9):e108253.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  24. Ninjurin1 deficiency attenuates susceptibility of experimental autoimmune encephalomyelitis in mice.
    Authors: Ahn, Bum Ju, Le, Hoang, Shin, Min Wook, Bae, Sung-Jin, Lee, Eun Ji, Wee, Hee-Jun, Cha, Jong-Ho, Lee, Hyo-Jong, Lee, Hye Shin, Kim, Jeong Hu, Kim, Chang-Ye, Seo, Ji Hae, Lo, Eng H, Jeon, Sejin, Lee, Mi-Ni, Oh, Goo Taeg, Yin, Guo Nan, Ryu, Ji-Kan, Suh, Jun-Kyu, Kim, Kyu-Won
    J Biol Chem, 2013-12-17;289(6):3328-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac.
    Authors: Vang, Amanda G, Housley, William, Dong, Hongli, Basole, Chaitali, Ben-Sasson, Shlomo Z, Kream, Barbara, Epstein, Paul M, Clark, Robert B, Brocke, Stefan
    Biochem J, 2013-12-15;456(3):463-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis.
    Authors: Nakamura, Takahiro, Hamuro, Junji, Takaishi, Mikiro, Simmons, Szandor, Maruyama, Kazuichi, Zaffalon, Andrea, Bentley, Adam J, Kawasaki, Satoshi, Nagata-Takaoka, Maho, Fullwood, Nigel J, Itami, Satoshi, Sano, Shigetos, Ishii, Masaru, Barrandon, Yann, Kinoshita, Shigeru
    J Clin Invest, 2013-12-09;124(1):385-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. TGF-beta promotes immune responses in the presence of mesenchymal stem cells.
    Authors: Xu C, Yu P, Han X, DU L, Gan J, Wang Y, Shi Y
    J Immunol, 2013-11-29;192(1):103-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  28. Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
    Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
    Virology, 2008-10-16;382(1):20-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. Identification of a murine CD28 dileucine motif that suppresses single-chain chimeric T-cell receptor expression and function.
    Authors: Nguyen P, Moisini I, Geiger TL
    Blood, 2003-08-28;102(13):4320-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay

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Reviews for Mouse CD3 Antibody

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Mouse CD3 Antibody
By Anonymous on 02/17/2022
Application: IHC Sample Tested: Spleen tissue Species: Mouse
Immunohistochemistry Mouse CD3 Antibody MAB4841
Mouse CD3 Antibody
By Anonymous on 08/13/2021
Application: IHC Sample Tested: Spleen tissue Species: Mouse
Immunohistochemistry Mouse CD3 Antibody MAB4841
Mouse CD3 Antibody
By Anonymous on 06/07/2018
Application: ICC/IF Sample Tested: Paraffined tumor tissue from HCC xenograft Species: Mouse

Green fluorescent is CD3 in mice tumors.

Immunocytochemistry/Immunofluorescence Mouse CD3 Antibody MAB4841
Mouse CD3 Antibody
By Anonymous on 05/16/2017
Application: ICC/IF Sample Tested: Adult heart Species: Mouse

Permeabilisation - 30mins at RT
Block -1% BSA - 30mins at RT
Primary Ab - diluted in block - 1 in 25 - for O/N at 4'C
Secondary ab - diluted in block - 2hrs at RT

Immunocytochemistry/Immunofluorescence Mouse CD3 Antibody MAB4841