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Mouse CD38 Antibody

Catalog #: AF4947
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4947
AF4947-SP
Detection of Mouse CD38 by Western Blot.
2 Images
Product Details
Citations (6)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD38 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD38 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross‑reactivity with recombinant human CD38 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD38
Leu45-Thr304
Accession # EDL37596
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CD38 antibody by Western Blot. View Larger

Detection of Mouse CD38 by Western Blot. Western blot shows lysates of mouse spleen tissue, mouse colon tissue, and mouse splenocytes. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse CD38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD38 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse CD38 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse CD38 by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for CD38 at approximately 64 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse CD38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4947) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD38

CD38, also known as ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability; cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta  cells (4, 5). CD38 has been shown to regulate oxytocin secretion, and may be involved in the development of complex social behaviors in mammals (6).

References
  1. Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
  2. Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
  3. Sun, L. et al. (1999) J. Cell Biol. 146:1161.
  4. Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
  5. Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
  6. Jin, D. et al. (2007) Nature 446:41.
Long Name
Cluster of Differentiation 38
Entrez Gene IDs
952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)
Alternate Names
2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20); 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl Cyclase; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase; cADPr hydrolase 1; CD_antigen: CD38; CD38 antigen (p45); CD38 antigen; CD38 molecule; CD38; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; Cyclic ADP-ribose Hydrolase; EC 2.4.99.20; EC 3.2.2.6; EC:3.2.2.6; NAD(+) nucleosidase; T10

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Citations for Mouse CD38 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells
    Authors: NR Nabar, CN Heijjer, CS Shi, IY Hwang, S Ganesan, MCI Karlsson, JH Kehrl
    Autophagy, 2021-07-27;0(0):19-Jan.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Senescent cells promote tissue NAD+ decline during ageing via the activation of CD38+ macrophages
    Authors: Anthony J. Covarrubias, Abhijit Kale, Rosalba Perrone, Jose Alberto Lopez-Dominguez, Angela Oliveira Pisco, Herbert G. Kasler et al.
    Nature Metabolism
  3. Deletion of CD38 Suppresses Glial Activation and Neuroinflammation in a Mouse Model of Demyelination
    Authors: Jureepon Roboon, Tsuyoshi Hattori, Hiroshi Ishii, Mika Takarada-Iemata, Thuong Manh Le, Yoshitake Shiraishi et al.
    Frontiers in Cellular Neuroscience
  4. The NADase CD38 is induced by factors secreted from senescent cells providing a potential link between senescence and age-related cellular NAD+ decline.
    Authors: Claudia C.S. Chini, Kelly A. Hogan, Gina M. Warner, Mariana G. Tarragó, Thais R. Peclat, Tamar Tchkonia et al.
    Biochemical and Biophysical Research Communications
  5. CD38 ecto-enzyme in immune cells is induced during aging and regulates NAD+ and NMN levels
    Authors: Chini CCS, Peclat TR, Warner GM et al.
    Nat Metab
  6. Endogenous metabolism in endothelial and immune cells generates most of the tissue vitamin B3 (nicotinamide)
    Authors: Zeidler J, Chini C, Kanamori K et al.
    iScience

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