Mouse CD6 Antibody

Catalog #: AF750
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF750
AF750-SP

All CD6 Antibodies

Product Details

Citations (1)

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Mouse CD6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD6 in direct ELISAs and Western blots. In Western blots, approximately 1% cross-reactivity with recombinant human CD6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD6
Leu18-Gly396
Accession # Q91WN5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD6 Fc Chimera (Catalog # 727-CD)

Adhesion Blockade

The adhesion of HuT 78 human cutaneous T cell lymphoma cells (5 x 10⁴ cells/well) to immobilized Recombinant Mouse CD6 Fc Chimera (Catalog # 727-CD, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 50 µg/mL of the antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD6

CD6 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 is a type I membrane glycoprotein and contains three extracellular SRCR domains. CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. Mouse CD6 is a 626 amino acid (aa) protein with a 24 aa signal sequence, a 372 aa extracellular domain, and a 204 aa cytoplasmic region. The 668 aa human homolog has also been identified. The human and murine proteins share 70% aa identity over their full lengths.

CD6 appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti‑CD2 mAb, support the concept of CD6 as a co-stimulatory molecule. Additionally, anti‑CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6⁺ T cells from donor bone marrow prior to allogenic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6, it has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development. Additionally, the presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6⁺ T cell and ALCAM⁺ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction. Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti‑CD6 mAb in conjunction with PMA, anti‑CD2, or anti‑CD3 mAb. The CD6 intracellular domain contains regions that can interact with SH2 or SH3 containing proteins.

References

Gangemi, R. et al. (1989) J. Immunol. 143:2439.
Aruffo, A. et al. (1991) J. Exp. Med. 174:949.
Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137.
Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765.
Whitney, G. et al. (1995) Mol. Immunol. 32:89.
Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738.
Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
Swack, J.A. et al. (1989) Mol. Immunol. 26:1037.
Pawelec, G. and H.J. Buhring (1991) Human Immunol. 31:165.
Osorio, L.M. et al. (1995) Cell Immunol. 166:44.
Robinson, W.H. et al. (1995) J. Immunol. 155:4739.
Singer, N.G. et al. (1996) Immunology 88:537.
Aruffo, A. et al. (1997) Immunol. Today 18:498.

Entrez Gene IDs

923 (Human); 12511 (Mouse); 102143944 (Cynomolgus Monkey)

Alternate Names

CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Mouse CD6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-kappaB
Authors: M Won, KA Park, S Kim, E Ju, Y Ko, H Yoo, H Ro, J Lee, J Oh, EG Lee, SY Kim, SW Nam, HM Shen, MK Yeo, JM Kim, GM Hur
Cell Death and Differentiation, 2021-11-27;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation