Mouse CD8 alpha Biotinylated Antibody

Catalog #: BAM116
2 Citations Datasheet / COA / SDS

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BAM116

Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry

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Citations (2)

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Mouse CD8 alpha Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD8 alpha. Reacts with alpha chains of mouse CD8 (Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579).

Source

Monoclonal Rat IgG_2A Clone # 53-6.7

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse thymus and spleen cells

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated CD8 alpha Monoclonal Antibody (Catalog # BAM116) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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CD8 alpha in Mouse Splenocytes. CD8a was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD8a Biotinylated Monoclonal Antibody (Catalog # BAM116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

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CD8 alpha in Mouse Splenocytes. CD8a was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CD8a Biotinylated Monoclonal Antibody (Catalog # BAM116) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References

Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
Hayakawa, K. et al. (1994) Science 263:1131.
MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
Rocha, B. et al. (1992) Immunol. Today 13:449.
Wang, J. and J.R. Klein (1994) Science 265:1860.
Vermec, D. et al. (1992) J. Exp. Med. 176:47.
Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Alternate Names

CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

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Citations for Mouse CD8 alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Cross-protective peptide vaccine against influenza A viruses developed in HLA-A*2402 human immunity model.
Authors: Ichihashi T, Yoshida R, Sugimoto C
PLoS ONE, 2011-09-19;6(9):e24626.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Blockade of transforming growth factor-{beta} signaling in tumor-reactive CD8(+) T cells activates the antitumor immune response cycle.
Authors: Zhang Q, Yang XJ, Kundu SD, Pins M, Javonovic B, Meyer R, Kim SJ, Greenberg NM, Kuzel T, Meagher R, Guo Y, Lee C
Mol. Cancer Ther., 2006-07-01;5(7):1733-43.
Species: Mouse
Sample Types: Whole Cells
Applications: Affinity Purification