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Mouse CD90/Thy1 Antibody

Catalog #: MAB7335
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7335
MAB7335-SP
Detection of Mouse CD90/Thy1 by Western Blot.
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Product Details
Citations (2)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CD90/Thy1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD90/Thy1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD90/Thy1 is observed.
Source
Monoclonal Rat IgG2A Clone # 778053
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse CD90/Thy1
Gln20-Lys130
Accession # P01831
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse CD90/Thy1 antibody by Western Blot. View Larger

Detection of Mouse CD90/Thy1 by Western Blot. Western blot shows lysates of mouse brain tissue and mouse thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CD90/Thy1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CD90/Thy1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD90/Thy1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD90/Thy1

CD90, also known as Thy1 (THYmocyte differentiation antigen 1) is a GPI-linked glycoprotein expressed by endothelial cells, hematopoietic stem cells, and in developing nervous tissue. Unlike human CD90, mouse CD90 is also highly expressed on T cells and is involved in T cell activation. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Entrez Gene IDs
7070 (Human); 21838 (Mouse)
Alternate Names
CD90 antigen; CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 membrane glycoprotein; Thy-1 T-cell antigen; Thy1

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Citations for Mouse CD90/Thy1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. An alphavbeta3 integrin checkpoint is critical for efficient TH2 cell cytokine polarization and potentiation of antigen-specific immunity
    Authors: ACH Szeto, ACF Ferreira, J Mannion, PA Clark, M Sivasubram, MWD Heycock, A Crisp, HE Jolin, P Kozik, MD Knolle, ANJ McKenzie
    Nature Immunology, 2022-12-22;24(1):123-135.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Establishment of in�vitro model of erectile dysfunction for the study of high-glucose-induced angiopathy and neuropathy
    Authors: G N Yin
    Andrology, 2016-12-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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