Mouse CDO Antibody

Catalog #: AF2429
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2429
AF2429-SP

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All CDO Antibodies

Product Details

Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CDO Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CDO in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CDO
Val22-Leu963
Accession # AAC43031

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (13.5-15.5 d.p.c.)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CDO by Western Blot. Western blot shows lysate of C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse CDO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2429) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CDO at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDO

Mouse CDO is a 190 kDa member of the Ig/FN-type III repeat subfamily of transmembrane (TM) proteins. The extracellular region contains five Ig-like domains and three FN-III modules. The molecule forms cis-complexes with itself and the related molecule termed BOC. Mouse CDO extracellular domain shares 95% and 85% aa identity with the corresponding regions of rat and human CDO, respectively.

Long Name

Cell Adhesion Molecule-related/Down-regulated by Oncogenes

Entrez Gene IDs

50937 (Human); 57810 (Mouse)

Alternate Names

CDO; Cdon homolog (mouse); CDON; CDOORCAM; cell adhesion molecule-related/down-regulated by oncogenes; MGC111524; ORCAM; surface glycoprotein, Ig superfamily member

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CDO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis
Authors: X Li, PJ Gordon, JA Gaynes, AW Fuller, R Ringuette, CP Santiago, V Wallace, S Blackshaw, P Li, EM Levine
Elife, 2022-12-02;11(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent Mï¿½ller glia
Authors: S Lim, YJ Kim, S Park, JH Choi, Y Sung, K Nishimori, Z Kozmik, HW Lee, JW Kim
Elife, 2021-10-22;10(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation
Authors: F Okumura, N Oki, Y Fujiki, R Ikuta, K Osaki, S Hamada, K Nakatsukas, N Hisamoto, T Hara, T Kamura
Scientific Reports, 2021-10-22;11(1):20880.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Dehydrocorydaline promotes myogenic differentiation via p38ï¿½MAPK activation
Mol Med Rep, 2016-08-19;14(4):3029-36.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Syntaxin 4 regulates the surface localization of a promyogenic receptor Cdo thereby promoting myogenic differentiation.
Authors: Yoo M, Kim B, Lee S, Jeong H, Park J, Seo D, Kim Y, Lee H, Han J, Kang J, Bae G
Skelet Muscle, 2015-09-07;5(0):28.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc.
Authors: Fabre PJ, Shimogori T, Charron F
J. Neurosci., 2010-01-06;30(0):266.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P