Mouse Chemerin Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 153 | 405 | 1124 | 168 | 385 | 1065 |
Standard Deviation | 13 | 16.2 | 99 | 14.2 | 25.7 | 64.1 |
CV% | 8.5 | 4 | 8.8 | 8.4 | 6.7 | 6 |
Recovery
The recovery of mouse Chemerin spiked to three levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Samples (n=4) | 102 | 89-117 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Chemerin
Chemerin, also known as Tazarotene-induced Gene 2, (Tig-2), is a member of the Cystatin superfamily. Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin fold-containing domain, and a six aa C-terminal prosegment. It is activated by proteases of the coagulation, fibrinolytic, and inflammatory cascades. Chemerin is a chemoattractant for cells bearing the Chem R23 receptor, including plasmacytoid dendritic cells and tissue macrophages.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
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Citations for Mouse Chemerin Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
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Dynamic and tissue-specific proteolytic processing of chemerin in obese mice
Authors: L Zhao, Y Yamaguchi, WJ Shen, J Morser, LLK Leung
PLoS ONE, 2018-08-30;13(8):e0202780.
Species: Mouse
Sample Types: Recombinant Protein
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Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia
Nat Commun, 2016-08-19;7(0):12528.
Species: Mouse
Sample Types: Cell Lysates
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Endothelial cell-derived chemerin promotes dendritic cell transmigration.
Authors: Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S
J Immunol, 2014-01-27;192(5):2366-73.
Species: Mouse
Sample Types: Cell Culture Supernates
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The chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses.
J. Exp. Med., 2012-07-02;209(8):1427-35.
Species: Mouse
Sample Types: Cell Culture Supernates
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Expression, Regulation, and Function of Atypical Chemerin Receptor CCRL2 on Endothelial Cells.
Authors: Monnier J, Lewen S, O'Hara E, Huang K, Tu H, Butcher EC, Zabel BA
J. Immunol., 2012-06-13;189(2):956-67.
Species: Mouse
Sample Types: Plasma
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Disruption of the chemokine-like receptor-1 (CMKLR1) gene is associated with reduced adiposity and glucose intolerance.
Authors: Ernst MC, Haidl ID, Zúñiga LA, Dranse HJ, Rourke JL, Zabel BA, Butcher EC, Sinal CJ
Endocrinology, 2011-12-20;153(2):672-82.
Species: Mouse
Sample Types: Serum
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ChemR23 dampens lung inflammation and enhances anti-viral immunity in a mouse model of acute viral pneumonia.
Authors: Bondue B, Vosters O, de Nadai P, Glineur S, De Henau O, Luangsay S, Van Gool F, Communi D, De Vuyst P, Desmecht D, Parmentier M
PLoS Pathog., 2011-11-03;7(11):e1002358.
Species: Mouse
Sample Types: Tissue Homogenates
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The Role of ChemR23 in the Induction and Resolution of Cigarette Smoke-Induced Inflammation.
Authors: Demoor T, Bracke KR, Dupont LL
J. Immunol., 2011-03-23;186(9):5457-67.
Species: Mouse
Sample Types: BALF
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Sterol regulatory element-binding protein 2 (SREBP2) activation after excess triglyceride storage induces chemerin in hypertrophic adipocytes.
Authors: Bauer S, Wanninger J, Schmidhofer S, Weigert J, Neumeier M, Dorn C, Hellerbrand C, Zimara N, Schaffler A, Aslanidis C, Buechler C
Endocrinology, 2010-11-17;152(1):26-35.
Species: Mouse
Sample Types: Cell Culture Supernates
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Expression of human chemerin induces insulin resistance in the skeletal muscle but does not affect weight, lipid levels, and atherosclerosis in LDL receptor knockout mice on high-fat diet.
Authors: Becker M, Rabe K, Lebherz C, Zugwurst J, Goke B, Parhofer KG, Lehrke M, Broedl UC
Diabetes, 2010-08-19;59(11):2898-903.
Species: Mouse
Sample Types: Serum
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