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Mouse Common  gamma  Chain/IL-2 R gamma Antibody

Catalog #: AF784
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF784
AF784-SP
Cell Proliferation Induced by IL‑15 and Neutralization by Mouse Common gamma Chain/ IL‑2 R gamma Antibody.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Common  gamma  Chain/IL-2 R gamma Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Common gamma Chain/IL‑2 R gamma in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) IL-2 R gamma is observed and less than 1% cross-reactivity with rhIL-2 R alpha, recombinant mouse IL-2 R beta, and rhIL-15 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Common gamma Chain/IL‑2 R gamma
Ser25-Ala263
Accession # Q3UPA9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Common  gamma  Chain/IL‑2 R gamma Fc Chimera (Catalog # 784-MR)
Neutralization
Measured by its ability to neutralize IL‑15-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 0.8 ng/mL Recombinant Human IL‑15.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑15 and Neutralization by Mouse Common gamma Chain/ IL‑2 R gamma Antibody. View Larger

Cell Proliferation Induced by IL‑15 and Neutralization by Mouse Common gamma Chain/ IL‑2 R gamma Antibody. Recombinant Human IL-15 (Catalog # 247-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Common gamma Chain/IL-2 R gamma Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF784). The ND50 is typically 6-24 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional mouse IL-2 receptor complex belongs to the hematopoietin receptor family. Mouse Common gamma Chain is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 241 residue extracellular domain, a 21 residue transmembrane domain and an 85 residue cytoplasmic domain. Although Common gamma Chain by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma  is also designated the common gamma chain ( gamma c) (1, 2). The site of molecular defects in human X-linked SCID (severe combined immunodeficiency) has been mapped to the human common gamma gene.

References
  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Noguchi, M. et al. (1993) Science 262:1877.
Long Name
IL-2 Receptor gamma
Entrez Gene IDs
3561 (Human); 16186 (Mouse)
Alternate Names
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

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Citations for Mouse Common  gamma  Chain/IL-2 R gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The Potential Role of a Soluble ?-Chain Cytokine Receptor as a Regulator of IL-7-Induced Lymphoproliferative Disorders
    Authors: G Kim, Y Jo, B Lee, LA Ali, B Lee, C Hong
    Int J Mol Sci, 2018-10-28;19(11):.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  2. A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells.
    Authors: Lai L, Zeff RA, Goldschneider I
    Blood, 2005-11-22;107(5):1776-84.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot

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