Mouse Complement Factor D/Adipsin Antibody Summary
Ile26-Ser259
Accession # P03953
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Mouse Complement Factor D/Adipsin antibody by Western Blot. Detection of Mouse Complement Factor D/Adipsin antibody by Western Blot.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Complement_Factor_D_AF5430_Western_Blot_5718.jpg)
Detection of Mouse Complement Factor D/Adipsin by Western Blot. Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor D/Adipsin at approximately 45kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor D/Adipsin
Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Factor D is 234 amino acids (aa) in length (aa 26‑259). In contains one peptidase S1 domain that cleaves an Arg-Lys bond (aa 26‑254). There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse factor D shares 85% and 68% aa identity with rat and human Factor D, respectively.
Product Datasheets
Citations for Mouse Complement Factor D/Adipsin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Selective targeting of visceral adiposity by polycation nanomedicine
Authors: Q Wan, B Huang, T Li, Y Xiao, Y He, W Du, BZ Wang, GF Dakin, M Rosenbaum, MD Goncalves, S Chen, KW Leong, L Qiang
Nature nanotechnology, 2022-12-01;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Adipsin-Dependent Secretion of Hepatocyte Growth Factor Regulates the Adipocyte-Cancer Stem Cell Interaction
Authors: M Mizuno, B Khaledian, M Maeda, T Hayashi, S Mizuno, E Munetsuna, T Watanabe, S Kono, S Okada, M Suzuki, S Takao, H Minami, N Asai, F Sugiyama, S Takahashi, Y Shimono
Cancers, 2021-08-23;13(16):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Adipsin promotes bone marrow adiposity by priming mesenchymal stem cells
Authors: N Aaron, MJ Kraakman, Q Zhou, Q Liu, S Costa, J Yang, L Liu, L Yu, L Wang, Y He, L Fan, H Hirakawa, L Ding, J Lo, W Wang, B Zhao, E Guo, L Sun, CJ Rosen, L Qiang
Elife, 2021-06-22;10(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina
Authors: D Pauly, D Agarwal, N Dana, N Schäfer, J Biber, KA Wunderlich, Y Jabri, T Straub, NR Zhang, AK Gautam, BHF Weber, SM Hauck, M Kim, CA Curcio, D Stambolian, M Li, A Grosche
Cell Rep, 2019-11-26;29(9):2835-2848.e4.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from Lipodystrophy
Authors: X Wu, I Hutson, AM Akk, S Mascharak, CTN Pham, DE Hourcade, R Brown, JP Atkinson, CA Harris
J. Immunol., 2018-03-12;0(0):.
Species: Mouse
Sample Types: Serum
Applications: Western Blot -
Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina
PLoS ONE, 2016-08-26;11(8):e0161898.
Species: Mouse
Sample Types: Plasma
Applications: Western Blot -
Complement factor B is the downstream effector of TLRs and plays an important role in a mouse model of severe sepsis.
Authors: Zou, Lin, Feng, Yan, Li, Yan, Zhang, Ming, Chen, Chan, Cai, Jiayan, Gong, Yu, Wang, Larry, Thurman, Joshua M, Wu, Xiaobo, Atkinson, John P, Chao, Wei
J Immunol, 2013-10-23;191(11):5625-35.
Species: Mouse
Sample Types: Serum
Applications: Western Blot
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