Mouse CTLA-4 Antibody

Catalog #: AF476
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF476
AF476-SP

All CTLA-4 Antibodies

Product Details

Citations (2)

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Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CTLA-4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CTLA-4 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human CTLA‑4, recombinant mouse (rm) CD28, rmICOS, and rmPD-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CTLA-4
Ala37-Phe162
Accession # Q6GTR6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CTLA‑4 Fc Chimera (Catalog # 434-CT)

Mouse CTLA-4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Mouse CTLA‑4 Biotinylated Antibody (Catalog # BAF476)

Standard: Recombinant Mouse CTLA-4 Fc Chimera Protein (Catalog # 434-CT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTLA-4

CTLA-4 and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. CTLA-4 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CTLA-4 and CD28 are composed of a single
Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CTLA-4 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2. CTLA-4 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CTLA-4 transcripts have since been found in both Th1 and Th2, and CD4⁺ and CD8⁺ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4⁺ T cells and 50% of CD8⁺ T cells and is down regulated upon T cell activation, CTLA-4 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with 20-100-fold higher affinity than CD28. The physiological role of CTLA-4 in T cell costimulation is currently being studied.

References

Lenschow, D.J. et al. (1996) Annu. Rev. Immunol. 14:233.
Hathcock, K.S. and R.J. Hodes (1996) Advances in Immunol. 62:131.
Ward, S.G. (1996) Biochem. J. 318:361.

Long Name

Cytotoxic T-lymphocyte-associated Molecule 4

Entrez Gene IDs

1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)

Alternate Names

CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CTLA-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis
Authors: W Deng, S Zhu, L Zeng, J Liu, R Kang, M Yang, L Cao, H Wang, TR Billiar, J Jiang, M Xie, D Tang
Cell Rep, 2018-07-10;24(2):366-378.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Marked prolongation of cardiac allograft survival by dendritic cells genetically engineered with NF-kappa B oligodeoxyribonucleotide decoys and adenoviral vectors encoding CTLA4-Ig.
Authors: Bonham CA, ž²¢6ï[, Peng L, Liang X, Chen Z, Wang L, Ma L, Hackstein H, Robbins PD, Thomson AW, Fung JJ, Qian S, Lu L
2002-09-15;169(6):3382-91.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development